ArticlePloS one2008
Rad51 polymerization reveals a new chromatin remodeling mechanism.
Article in PloS one, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 33 citations in OpenAlex.
- Homologous Recombination and DNA Intermediates Analyzed by Electron Microscopy.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Article
- FIGNL1 AAA+ ATPase remodels RAD51 and DMC1 filaments in pre-meiotic DNA replication and meiotic recombination.Nature communications · 2023Article
- Method combining BAC film and positive staining for the characterization of DNA intermediates by dark-field electron microscopy.Biology methods & protocols · 2020Article
- In vitro role of Rad54 in Rad51-ssDNA filament-dependent homology search and synaptic complexes formation.Nature communications · 2019Article
- Rouse model with transient intramolecular contacts on a timescale of seconds recapitulates folding and fluctuation of yeast chromosomes.Nucleic acids research · 2019Article
- C-terminal region of bacterial Ku controls DNA bridging, DNA threading and recruitment of DNA ligase D for double strand breaks repair.Nucleic acids research · 2016Article
- Dual and Opposite Effects of hRAD51 Chemical Modulation on HIV-1 Integration.Chemistry & biology · 2015Article
- ATP-dependent nucleosome unwrapping catalyzed by human RAD51.Nucleic acids research · 2013Article
- Multiple binding of repressed mRNAs by the P-body protein Rck/p54.RNA (New York, N.Y.) · 2012Article
- Basic concepts of epigenetics: impact of environmental signals on gene expression.Epigenetics · 2012Review
- Article
- Stimulation of the human RAD51 nucleofilament restricts HIV-1 integration in vitro and in infected cells.Journal of virology · 2012Article
- Histone fold modifications control nucleosome unwrapping and disassembly.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Nucleosome remodeling by hMSH2-hMSH6.Molecular cell · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rad51 protein is a well known protagonist of homologous recombination in eukaryotic cells. Rad51 polymerization on single-stranded DNA and its role in presynaptic filament formation have been extensively documented. Rad51 polymerizes also on double-stranded DNA but the significance of this filament formation remains unclear. We explored the behavior of Saccharomyces cerevisiae Rad51 on dsDNA and the influence of nucleosomes on Rad51 polymerization mechanism to investigate its putative role in chromatin accessibility to recombination machinery. We combined biochemical approaches, transmission electron microscopy (TEM) and atomic force microscopy (AFM) for analysis of the effects of the Rad51 filament on chromatinized templates. Quantitative analyses clearly demonstrated the occurrence of chromatin remodeling during nucleoprotein filament formation. During Rad51 polymerization, recombinase proteins moved all the nucleosomal arrays in front of the progressing filament. This polymerization process had a powerful remodeling effect, as Rad51 destabilized the nucleosomes along considerable stretches of DNA. Similar behavior was observed with RecA. Thus, recombinase polymerization is a powerful mechanism of chromatin remodeling. These remarkable features open up new possibilities for understanding DNA recombination and reveal new types of ATP-dependent chromatin dynamics.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.