ArticleMolecular and cellular biology2009
Conservation of the protein composition and electron microscopy structure of Drosophila melanogaster and human spliceosomal complexes.
Article in Molecular and cellular biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 106 papers.
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Who cites it
106 citing papers in PubMed, 176 citations in OpenAlex.
- Article
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- Genetic and genomic analyses of Drosophila melanogaster models of chromatin modification disorders.Genetics · 2023Article
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- The DEAD-box RNA helicase Dhx15 controls glycolysis and arbovirus replication in Aedes aegypti mosquito cells.PLoS pathogens · 2022Article
- A genetic screen in C. elegans reveals roles for KIN17 and PRCC in maintaining 5' splice site identity.PLoS genetics · 2022Article
- An exon junction complex-independent function of Barentsz in neuromuscular synapse growth.EMBO reports · 2022Article
- Nucleotide Variation inFrontiers in physiology · 2022Article
- SPF45/RBM17-dependent, but not U2AF-dependent, splicing in a distinct subset of human short introns.Nature communications · 2021Article
- A functional genomics screen identifying blood cell development genes in Drosophila by undergraduates participating in a course-based research experience.G3 (Bethesda, Md.) · 2021Article
- Multi-responses of O-methyltransferase genes to salt stress and fiber development of Gossypium species.BMC plant biology · 2021Article
- Capturing the transcription factor interactome in response to sub-lethal insecticide exposure.Current research in insect science · 2021Article
- NineTeen Complex-subunit Salsa is required for efficient splicing of a subset of introns and dorsal-ventral patterning.RNA (New York, N.Y.) · 2020Article
- Loss of the RNA trimethylguanosine cap is compatible with nuclear accumulation of spliceosomal snRNAs but not pre-mRNA splicing or snRNA processing during animal development.PLoS genetics · 2020Article
46 more citing papers are in PubMed but not listed here.
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6 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Comprehensive proteomics analyses of spliceosomal complexes are currently limited to those in humans, and thus, it is unclear to what extent the spliceosome's highly complex composition and compositional dynamics are conserved among metazoans. Here we affinity purified Drosophila melanogaster spliceosomal B and C complexes formed in Kc cell nuclear extract. Mass spectrometry revealed that their composition is highly similar to that of human B and C complexes. Nonetheless, a number of Drosophila-specific proteins were identified, suggesting that there may be novel factors contributing specifically to splicing in flies. Protein recruitment and release events during the B-to-C transition were also very similar in both organisms. Electron microscopy of Drosophila B complexes revealed a high degree of structural similarity with human B complexes, indicating that higher-order interactions are also largely conserved. A comparison of Drosophila spliceosomes formed on a short versus long intron revealed only small differences in protein composition but, nonetheless, clear structural differences under the electron microscope. Finally, the characterization of affinity-purified Drosophila mRNPs indicated that exon junction complex proteins are recruited in a splicing-dependent manner during C complex formation. These studies provide insights into the evolutionarily conserved composition and structure of the metazoan spliceosome, as well as its compositional dynamics during catalytic activation.
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