ArticleThe Journal of biological chemistry2009
Retinoblastoma protein (pRb), but not p107 or p130, is required for maintenance of enterocyte quiescence and differentiation in small intestine.
Article in The Journal of biological chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
What it found
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Who cites it
52 citing papers in PubMed, 61 citations in OpenAlex.
- Protocol for iterative indirect immunofluorescence imaging of frozen mouse intestinal tissues.STAR protocols · 2026Article
- Retinoblastoma Protein Paralogs and Tumor Suppression.Frontiers in genetics · 2022Review
- Reprogramming of H3K9bhb at regulatory elements is a key feature of fasting in the small intestine.Cell reports · 2021Article
- Compensation between Wnt-driven tumorigenesis and cellular responses to ribosome biogenesis inhibition in the murine intestinal epithelium.Cell death and differentiation · 2020Article
- Serum deprivation initiates adaptation and survival to oxidative stress in prostate cancer cells.Scientific reports · 2020Article
- EGFR in enterocytes & endothelium and HIF1α in enterocytes are dispensable for massive small bowel resection induced angiogenesis.PloS one · 2020Article
- Spliceosome protein Eftud2 promotes colitis-associated tumorigenesis by modulating inflammatory response of macrophage.Mucosal immunology · 2019Article
- Article
- Intestinal epithelial cell-specific Raptor is essential for high fat diet-induced weight gain in mice.Biochemical and biophysical research communications · 2018Article
- An In Vitro Model of Cellular Quiescence in Primary Human Dermal Fibroblasts.Methods in molecular biology (Clifton, N.J.) · 2018Article
- Epithelial cell specific Raptor is required for initiation of type 2 mucosal immunity in small intestine.Scientific reports · 2017Article
- Toll-like receptor 4 is critical for the development of resection-associated hepatic steatosis.Journal of pediatric surgery · 2017Article
- Epithelial IGF1R is dispensable for IGF2 mediated enhanced intestinal adaptation in retinoblastoma-deficient mice.Journal of pediatric surgery · 2017Article
- GATA4 Is Sufficient to Establish Jejunal Versus Ileal Identity in the Small Intestine.Cellular and molecular gastroenterology and hepatology · 2017Article
- Intestinal Epithelial-Specific mTORC1 Activation Enhances Intestinal Adaptation After Small Bowel Resection.Cellular and molecular gastroenterology and hepatology · 2017Article
- Article
- Inhibition of CDK4/6 protects against radiation-induced intestinal injury in mice.The Journal of clinical investigation · 2016Article
- The Pathogenesis of Resection-Associated Intestinal Adaptation.Cellular and molecular gastroenterology and hepatology · 2016Review
- Article
- Fasting protects mice from lethal DNA damage by promoting small intestinal epithelial stem cell survival.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
The function of retinoblastoma protein (pRb) in the regulation of small intestine epithelial cell homeostasis has been challenged by several groups using various promoter-based Cre transgenic mouse lines. Interestingly, different pRb deletion systems yield dramatically disparate small intestinal phenotypes. These findings confound the function of pRb in this dynamic tissue. In this study, Villin-Cre transgenic mice were crossed with Rb (flox/flox) mice to conditionally delete pRb protein in small intestine enterocytes. We discovered a novel hyperplasia phenotype as well as ectopic cell cycle reentry within villus enterocytes in the small intestine. This phenotype was not seen in other pRb family member (p107 or p130) null mice. Using a newly developed crypt/villus isolation method, we uncovered that expression of pRb was undetectable, whereas proliferating cell nuclear antigen, p107, cyclin E, cyclin D3, Cdk2, and Cdc2 were dramatically increased in pRb-deficient villus cells. Cyclin A, cyclin D1, cyclin D2, and Cdk4/6 expression was not affected by absent pRb expression. pRb-deficient villus cells appeared capable of progressing to mitosis but with higher rates of apoptosis. However, the cycling villus enterocytes were not completely differentiated as gauged by significant reduction of intestinal fatty acid-binding protein expression. In summary, pRb, but not p107 or p130, is required for maintaining the postmitotic villus cell in quiescence, governing the expression of cell cycle regulatory proteins, and completing of absorptive enterocyte differentiation in the small intestine.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.