ArticleBMC genomics2008
mtDNA depletion confers specific gene expression profiles in human cells grown in culture and in xenograft.
Article in BMC genomics, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
28 citing papers in PubMed, 56 citations in OpenAlex.
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- Transcriptomic analysis of cells following decreased mitochondrial DNA-copy number reveals compensatory mechanisms in mitochondrial DNA replication and cellular energetics.bioRxiv : the preprint server for biology · 2025Article
- Mitochondrial DNA Deficiency and Supplementation inInternational journal of molecular sciences · 2023Article
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- Mitochondrial DNA Affects the Expression of Nuclear Genes Involved in Immune and Stress Responses in a Breast Cancer Model.Frontiers in physiology · 2020Article
- The natural compound gracillin exerts potent antitumor activity by targeting mitochondrial complex II.Cell death & disease · 2019Article
- The SIAH2-NRF1 axis spatially regulates tumor microenvironment remodeling for tumor progression.Nature communications · 2019Article
- Intracellular oxygen mapping using a myoglobin-mCherry probe with fluorescence lifetime imaging.Journal of biomedical optics · 2018Article
- Dissecting the expression landscape of mitochondrial genes in lung squamous cell carcinoma and lung adenocarcinoma.Oncology letters · 2018Article
- Aggressive triple negative breast cancers have unique molecular signature on the basis of mitochondrial genetic and functional defects.Biochimica et biophysica acta. Molecular basis of disease · 2018Article
- Oncogene-Driven Metabolic Alterations in Cancer.Biomolecules & therapeutics · 2018Review
- Mitochondrial determinants of cancer health disparities.Seminars in cancer biology · 2017Review
- Mitochondrial dysfunction and mitochondrial dynamics-The cancer connection.Biochimica et biophysica acta. Bioenergetics · 2017Review
- Mitochondrial DNA content in breast cancer: Impact on in vitro and in vivo phenotype and patient prognosis.Oncotarget · 2016Article
- Cross Talk of Proteostasis and Mitostasis in Cellular Homeodynamics, Ageing, and Disease.Oxidative medicine and cellular longevity · 2016Review
- Induced pluripotent stem cell models of Zellweger spectrum disorder show impaired peroxisome assembly and cell type-specific lipid abnormalities.Stem cell research & therapy · 2015Article
- Mitochondrial retrograde signaling induces epithelial-mesenchymal transition and generates breast cancer stem cells.Oncogene · 2014Article
- Cancer metabolic reprogramming: importance, main features, and potentials for precise targeted anti-cancer therapies.Cancer biology & medicine · 2014Review
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundInteractions between the gene products encoded by the mitochondrial and nuclear genomes play critical roles in eukaryotic cellular function. However, the effects mitochondrial DNA (mtDNA) levels have on the nuclear transcriptome have not been defined under physiological conditions. In order to address this issue, we characterized the gene expression profiles of A549 lung cancer cells and their mtDNA-depleted rho0 counterparts grown in culture and as tumor xenografts in immune-deficient mice.
resultsCultured A549 rho0 cells were respiration-deficient and showed enhanced levels of transcripts relevant to metal homeostasis, initiation of the epithelial-mesenchymal transition, and glucuronidation pathways. Several well-established HIF-regulated transcripts showed increased or decreased abundance relative to the parental cell line. Furthermore, growth in culture versus xenograft has a significantly greater influence on expression profiles, including transcripts involved in mitochondrial structure and both aerobic and anaerobic energy metabolism. However, both in vitro and in vivo, mtDNA levels explained the majority of the variance observed in the expression of transcripts in glucuronidation, tRNA synthetase, and immune surveillance related pathways. mtDNA levels in A549 xenografts also affected the expression of genes, such as AMACR and PHYH, involved in peroxisomal lipid metabolic pathways.
conclusionWe have identified mtDNA-dependent gene expression profiles that are shared in cultured cells and in xenografts. These profiles indicate that mtDNA-depleted cells could provide informative model systems for the testing the efficacy of select classes of therapeutics, such as anti-angiogenesis agents. Furthermore, mtDNA-depleted cells grown culture and in xenografts provide a powerful means to investigate possible relationships between mitochondrial activity and gene expression profiles in normal and pathological cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.