ArticleBMC immunology2008
Study of membrane potential in T lymphocytes subpopulations using flow cytometry.
Article in BMC immunology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 29 citations in OpenAlex.
- Novel method for kinetic analysis applied to transport by the uniporter OCT2.American journal of physiology. Renal physiology · 2022Article
- Electroimmunology and cardiac arrhythmia.Nature reviews. Cardiology · 2021Review
- Silver nanoparticles and silver ions cause inflammatory response through induction of cell necrosis and the release of mitochondria in vivo and in vitro.Cell biology and toxicology · 2021Article
- Inactivation of TRPM7 kinase in mice results in enlarged spleens, reduced T-cell proliferation and diminished store-operated calcium entry.Scientific reports · 2018Article
- Differential Activity of Voltage- and CaFrontiers in physiology · 2018Article
- Endogenous Bioelectric Signaling Networks: Exploiting Voltage Gradients for Control of Growth and Form.Annual review of biomedical engineering · 2017Review
- The functional contribution of calcium ion flux heterogeneity in T cells.Immunology and cell biology · 2015Review
- The CNS under pathophysiologic attack--examining the role of K₂p channels.Pflugers Archiv : European journal of physiology · 2015Review
- Cationic nanocarriers induce cell necrosis through impairment of Na(+)/K(+)-ATPase and cause subsequent inflammatory response.Cell research · 2015Article
- Placing ion channels into a signaling network of T cells: from maturing thymocytes to healthy T lymphocytes or leukemic T lymphoblasts.BioMed research international · 2015Review
- Molecular bioelectricity: how endogenous voltage potentials control cell behavior and instruct pattern regulation in vivo.Molecular biology of the cell · 2014Article
- Kinetic measurements using flow cytometry: new methods for monitoring intracellular processes.Assay and drug development technologies · 2012Article
- Kv1.5 in the immune system: the good, the bad, or the ugly?Frontiers in physiology · 2010Article
- The functional network of ion channels in T lymphocytes.Immunological reviews · 2009Review
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundIon channels are involved in the control of membrane potential (psi) in a variety of cells. The maintenance of psi in human T lymphocytes is essential for T-cell activation and was suggested to depend mostly on the voltage-gated Kv1.3 channel. Blockage of Kv1.3 inhibits cytokine production and lymphocyte proliferation in vitro and suppresses immune response in vivo. T lymphocytes are a heterogeneous cell population and the expression of Kv1.3 varies among cell subsets. Oxonol diBA-C4-(3) was used to determine psi by flow cytometry. The presence of distinct T cell subsets was evaluated by immunophenotyping techniques and the contribution of Kv1.3 channels for the maintenance of psi was investigated using selective blockers.
resultsThe distribution of psi in T lymphocytes varied among blood donors and did not always follow a unimodal pattern. T lymphocytes were divided into CD3+/CD45RO- and CD3+/CD45RO+ subsets, whose peak channel values of psi were -58 +/- 3.6 mV and -37 +/- 4.1 mV, respectively. MgTX (specific inhibitor of Kv1.3 channels) had no significant effect in the psi of CD3+/CD45RO- subsets but depolarized CD3+/CD45RO+ cells to -27 +/- 5.1 mV.
conclusionCombination of optical methods for determination of psi by flow cytometry with immuophenotyping techniques opens new possibilities for the study of ion channels in the biology of heterogeneous cell populations such as T lymphocyte subsets.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.