ArticleBMC genetics2008
A strategy to setup codominant microsatellite analysis for high-resolution-melting-curve-analysis (HRM).
Article in BMC genetics, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers, 1 of them a synthesis that pooled it.
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Who cites it
14 citing papers in PubMed, 1 synthesis or guideline pooled it, 51 citations in OpenAlex.
- Rapid and cost-effective screening of newly identified microsatellite loci by high-resolution melting analysis.Molecular genetics and genomics : MGG · 2011Pooled it
- Article
- High-resolution melt (HRM) analysis for detection of SNPs associated with pyrethroid resistance in the southern cattle fever tick, Rhipicephalus (Boophilus) microplus (Acari: Ixodidae).International journal for parasitology. Drugs and drug resistance · 2019Article
- Sensitive High-Resolution Melting Analysis for Screening of KRAS and BRAF Mutations in Iranian Human Metastatic Colorectal CancersAsian Pacific journal of cancer prevention : APJCP · 2016Article
- DNA barcode ITS2 coupled with high resolution melting (HRM) analysis for taxonomic identification of Sideritis species growing in Greece.Molecular biology reports · 2014Article
- Pathotype-specific QTL for stem rust resistance in Lolium perenne.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2013Article
- Excised radicle tips as a source of genomic DNA for PCR-based genotyping and melting curve analysis in cotton.Journal of biosciences · 2013Article
- Analysis of strain relatedness using high resolution melting in a case of recurrent candiduria.BMC microbiology · 2013Article
- Article
- Genetic mapping of paternal sorting of mitochondria in cucumber.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2012Article
- Article
- Identification of forensically important blowfly species (Diptera: Calliphoridae) by high-resolution melting PCR analysis.International journal of legal medicine · 2010Article
- High-resolution melting analysis of cDNA-derived PCR amplicons for rapid and cost-effective identification of novel alleles in barley.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2009Article
- High-resolution melting-based TILLING of γ ray-induced mutations in rice.Journal of Zhejiang University. Science. BArticle
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundHigh resolution melting curve analysis (HRM) is a technique that measures exactly the decreasing fluorescence of intercalating dye in the process of dissociation of double stranded DNA. The measurement is immediately following PCR in a one-step, closed-tube method. The shape of the melting curve depends on the GC content, length and sequence of the amplicon. Hence it is a powerful, fast and cheap method to detect Single Nucleotide Polymorphisms (SNPs) and other mutations.
resultsHere we present a strategy to set up microsatellite analysis for HRM including the correct assignment of heterozygous samples by comparative analysis and artificial mixtures of samples. The approach is demonstrated on two Simple Sequence Repeat (SSR) loci of different complexity in the genus Origanum. Following this strategy all alleles of our sample sets could be classified correctly.
conclusionHRM can be used in microsatellite analysis and other codominant marker systems implementing a protocol of comparative melting curve assignment with artificial mixtures of samples to overcome difficulties in correctly assigning heterozygous samples. The method is faster, more sensitive and cheaper than standard protocols for microsatellite analysis.
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