Evidence map›Paper›PMID 18979230›Full record

ArticleMolecular biology reports2009

Genetic variation and population structure of endemic yellow catfish, Horabagrus brachysoma (Bagridae) among three populations of Western Ghat region using RAPD and microsatellite markers.

P M A Muneer, A Gopalakrishnan, K K Musammilu, Vindhya Mohindra, K K Lal, V S Basheer, W S Lakra

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Article in Molecular biology reports, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.

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17citing papers in PubMed
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3 · Its place in the literature

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17 citing papers in PubMed.

  1. Article
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5 · Who and what money

Authors and funding

7 authors.

P M A MuneerNational Bureau of Fish Genetic Resources, Cochin Unit, CMFRI Campus, Cochin, Kerala, India. pmamuneer@gmail.com
A Gopalakrishnan
K K Musammilu
Vindhya Mohindra
K K Lal
V S Basheer
W S Lakra

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Random amplified polymorphic DNA (RAPD) and microsatellite markers were applied to evaluate the genetic variation in endemic and endangered yellow catfish, Horabagrus brachysoma sampled from three geographic locations of Western Ghat, South India river systems. In RAPD, of 32 10-mer RAPD primers screened initially, 10 were chosen and used in a comparative analysis of H. brachysoma collected from Meenachil, Chalakkudy and Nethravathi River systems. Of the 124 total RAPD fragments amplified, 49 (39.51%) were found to be shared by individuals of all 3 populations. The remaining 75 fragments were found to be polymorphic (60.48%). In microsatellites, six polymorphic microsatellite loci were identified by using primers developed for Pangasius hypophthalmus, Clarias macrocephalus and Clarias gariepinus. The identified loci were confirmed as microsatellite by sequencing after making a clone. The nucleotide sequences of 6 loci were published in NCBI genbank. The number of alleles across the six loci ranged from 4 to 7 and heterozygosities ranged from 0.07 to 0.93. The mean number of alleles and effective number of alleles per locus were 5.00 and 3.314, respectively. The average heterozygosity across all investigated samples was 0.72, indicating a significant deficiency of heterozygotes in this species. RAPD and microsatellite methods reported a high degree of gene diversity and genetic distances depicted by UPGMA dendrograms among the populations of H. brachysoma.

Indexed as

EcosystemGenetic VariationRandom Amplified Polymorphic DNA TechniqueAllelesAnimalsBase SequenceCatfishesGene FlowGenetic MarkersGeographyInbreedingIndiaMicrosatellite RepeatsMolecular Sequence DataPhylogenyPopulation DynamicsGenetic Markers

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.