ArticlePloS one2008
Identification of Spt5 target genes in zebrafish development reveals its dual activity in vivo.
Article in PloS one, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 38 citations in OpenAlex.
- NusG-Spt5 Transcription Factors: Universal, Dynamic Modulators of Gene Expression.Journal of molecular biology · 2025Review
- The transcription elongation factors Spt4 and Spt5 control neural progenitor proliferation and are implicated in neuronal remodeling duringFrontiers in cell and developmental biology · 2024Article
- The hemoglobinopathies, molecular disease mechanisms and diagnostics.International journal of laboratory hematology · 2022Review
- SPT6-driven error-free DNA repair safeguards genomic stability of glioblastoma cancer stem-like cells.Nature communications · 2020Article
- Widespread transcriptional pausing and elongation control at enhancers.Genes & development · 2018Article
- Spt5 Plays Vital Roles in the Control of Sense and Antisense Transcription Elongation.Molecular cell · 2017Article
- MVIAeval: a web tool for comprehensively evaluating the performance of a new missing value imputation algorithm.BMC bioinformatics · 2017Article
- Increased functional protein expression using nucleotide sequence features enriched in highly expressed genes in zebrafish.Nucleic acids research · 2015Article
- The transcript elongation factor SPT4/SPT5 is involved in auxin-related gene expression in Arabidopsis.Nucleic acids research · 2014Article
- Pausing for thought: disrupting the early transcription elongation checkpoint leads to developmental defects and tumourigenesis.BioEssays : news and reviews in molecular, cellular and developmental biology · 2013Article
- Article
- Uncovering hidden duplicated content in public transcriptomics data.Database : the journal of biological databases and curation · 2013Article
- Zebrafish chemical screening reveals the impairment of dopaminergic neuronal survival by cardiac glycosides.PloS one · 2012Article
- Differentially regulated splice variants and systems biology analysis of Kaposi's sarcoma-associated herpesvirus-infected lymphatic endothelial cells.Nucleic acids research · 2011Article
- Genomic selection identifies vertebrate transcription factor Fezf2 binding sites and target genes.The Journal of biological chemistry · 2011Article
- Article
- Cotranscriptional recruitment of She2p by RNA pol II elongation factor Spt4-Spt5/DSIF promotes mRNA localization to the yeast bud.Genes & development · 2010Article
- Article
- AltAnalyze and DomainGraph: analyzing and visualizing exon expression data.Nucleic acids research · 2010Article
- Alternative splicing regulates mouse embryonic stem cell pluripotency and differentiation.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Spt5 is a conserved essential protein that represses or stimulates transcription elongation in vitro. Immunolocalization studies on Drosophila polytene chromosomes suggest that Spt5 is associated with many loci throughout the genome. However, little is known about the prevalence and identity of Spt5 target genes in vivo during development. Here, we identify direct target genes of Spt5 using fog(sk8) zebrafish mutant, which disrupts the foggy/spt5 gene. We identified that fog(sk8) and their wildtype siblings differentially express less than 5% of genes examined. These genes participate in diverse biological processes from stress response to cell fate specification. Up-regulated genes exhibit shorter overall gene length compared to all genes examined. Through chromatin immunoprecipitation in zebrafish embryos, we identified a subset of developmentally critical genes that are bound by both Spt5 and RNA polymerase II. The protein occupancy patterns on these genes are characteristic of both repressive and stimulatory elongation regulation. Together our findings establish Spt5 as a dual regulator of transcription elongation in vivo and identify a small but diverse set of target genes critically dependent on Spt5 during development.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.