ArticleApplied and environmental microbiology2009
Specific detection of viable Legionella cells by combined use of photoactivated ethidium monoazide and PCR/real-time PCR.
Article in Applied and environmental microbiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
16 citing papers in PubMed, 68 citations in OpenAlex.
- Development of a PCR Assay for the Detection ofInfectious disease reports · 2025Article
- The Impact of Storms onFrontiers in microbiology · 2020Article
- PCR method for the rapid detection and discrimination of Legionella spp. based on the amplification of pcs, pmtA, and 16S rRNA genes.Journal of applied genetics · 2016Article
- Determination of viable legionellae in engineered water systems: Do we find what we are looking for?Water research · 2016Review
- Experimental design for the optimization of propidium monoazide treatment to quantify viable and non-viable bacteria in piggery effluents.BMC microbiology · 2015Article
- Molecular characterization of viable Legionella spp. in cooling tower water samples by combined use of ethidium monoazide and PCR.Microbes and environments · 2015Article
- Current and emerging Legionella diagnostics for laboratory and outbreak investigations.Clinical microbiology reviews · 2015Review
- Detection of Legionella, L. pneumophila and Mycobacterium avium complex (MAC) along potable water distribution pipelines.International journal of environmental research and public health · 2014Article
- Mycobacterium avium complex in day care hot water systems, and persistence of live cells and DNA in hot water pipes.Current microbiology · 2014Article
- Uncertainties associated with assessing the public health risk from Legionella.Frontiers in microbiology · 2014Review
- Application of quantitative PCR for the detection of microorganisms in water.Analytical and bioanalytical chemistry · 2013Review
- Application of EMA-qPCR as a complementary tool for the detection and monitoring of Legionella in different water systems.World journal of microbiology & biotechnology · 2012Article
- Specific real-time PCR for simultaneous detection and identification of Legionella pneumophila serogroup 1 in water and clinical samples.Applied and environmental microbiology · 2011Article
- Specific and rapid enumeration of viable but nonculturable and viable-culturable gram-negative bacteria by using flow cytometry.Applied and environmental microbiology · 2010Article
- Opportunistic pathogens enriched in showerhead biofilms.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Viability PCR, a culture-independent method for rapid and selective quantification of viable Legionella pneumophila cells in environmental water samples.Applied and environmental microbiology · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Legionella organisms are prevalent in manmade water systems and cause legionellosis in humans. A rapid detection method for viable Legionella cells combining ethidium monoazide (EMA) and PCR/real-time PCR was assessed. EMA could specifically intercalate and cleave the genomic DNA of heat- and chlorine-treated dead Legionella cells. The EMA-PCR assay clearly showed an amplified fragment specific for Legionella DNA from viable cells, but it could not do so for DNA from dead cells. The number of EMA-treated dead Legionella cells estimated by real-time PCR exhibited a 10(4)- to 10(5)-fold decrease compared to the number of dead Legionella cells without EMA treatment. Conversely, no significant difference in the numbers of EMA-treated and untreated viable Legionella cells was detected by the real-time PCR assay. The combined assay was also confirmed to be useful for specific detection of culturable Legionella cells from water samples obtained from spas. Therefore, the combined use of EMA and PCR/real-time PCR detects viable Legionella cells rapidly and specifically and may be useful in environmental surveillance for Legionella.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.