ArticleJournal of bacteriology2009
Type II secretion system secretin PulD localizes in clusters in the Escherichia coli outer membrane.
Article in Journal of bacteriology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 47 citations in OpenAlex.
- Modularization of the type II secretion gene cluster from Xanthomonas euvesicatoria facilitates the identification of a structurally conserved XpsCLM assembly platform complex.PLoS pathogens · 2025Article
- Nanofitins and their applications in human health and lung diseases.Molecular therapy : the journal of the American Society of Gene Therapy · 2023Article
- Downsizing a pullulanase to a small molecule with improved soluble expression and secretion efficiency in Escherichia coli.Microbial cell factories · 2016Article
- Artificial affinity proteins as ligands of immunoglobulins.Biomolecules · 2015Review
- Article
- Outside-in assembly pathway of the type IV pilus system in Myxococcus xanthus.Journal of bacteriology · 2014Article
- Potent and specific inhibition of glycosidases by small artificial binding proteins (affitins).PloS one · 2014Article
- Article
- Absence of long-range diffusion of OmpA in E. coli is not caused by its peptidoglycan binding domain.BMC microbiology · 2013Article
- Outer membrane targeting, ultrastructure, and single molecule localization of the enteropathogenic Escherichia coli type IV pilus secretin BfpB.Journal of bacteriology · 2012Article
- Structural insights into the Type II secretion nanomachine.Current opinion in structural biology · 2012Review
- Secretins: dynamic channels for protein transport across membranes.Trends in biochemical sciences · 2011Review
- Deciphering the assembly of the Yersinia type III secretion injectisome.The EMBO journal · 2010Article
- Molecular mechanisms of enterotoxigenic Escherichia coli infection.Microbes and infection · 2010Review
- The essential Escherichia coli apolipoprotein N-acyltransferase (Lnt) exists as an extracytoplasmic thioester acyl-enzyme intermediate.Biochemistry · 2010Article
- Legionella pneumophila secretes an endoglucanase that belongs to the family-5 of glycosyl hydrolases and is dependent upon type II secretion.FEMS microbiology letters · 2009Article
- Many substrates and functions of type II secretion: lessons learned from Legionella pneumophila.Future microbiology · 2009Review
- Docking and assembly of the type II secretion complex of Vibrio cholerae.Journal of bacteriology · 2009Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The cellular localization of a chimera formed by fusing a monomeric red fluorescent protein to the C terminus of the Klebsiella oxytoca type II secretion system outer membrane secretin PulD (PulD-mCherry) in Escherichia coli was determined in vivo by fluorescence microscopy. Like PulD, PulD-mCherry formed sodium dodecyl sulfate- and heat-resistant multimers and was functional in pullulanase secretion. Chromosome-encoded PulD-mCherry formed fluorescent foci on the periphery of the cell in the presence of high (plasmid-encoded) levels of its cognate chaperone, the pilotin PulS. Subcellular fractionation demonstrated that the chimera was located exclusively in the outer membrane under these circumstances. A similar localization pattern was observed by fluorescence microscopy of fixed cells treated with green fluorescent protein-tagged affitin, which binds with high affinity to an epitope in the N-terminal region of PulD. At lower levels of (chromosome-encoded) PulS, PulD-mCherry was less stable, was located mainly in the inner membrane, from which it could not be solubilized with urea, and did not induce the phage shock response, unlike PulD in the absence of PulS. The fluorescence pattern of PulD-mCherry under these conditions was similar to that observed when PulS levels were high. The complete absence of PulS caused the appearance of bright and almost exclusively polar fluorescent foci.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.