ArticleJournal of bacteriology2009
The pH-responsive regulon of HP0244 (FlgS), the cytoplasmic histidine kinase of Helicobacter pylori.
Article in Journal of bacteriology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
28 citing papers in PubMed, 1 synthesis or guideline pooled it, 47 citations in OpenAlex.
- Infiltration to infection: key virulence players of Helicobacter pylori pathogenicity.Infection · 2024Pooled it
- Article
- Large-scale phylogenomics of aquatic bacteria reveal molecular mechanisms for adaptation to salinity.Science advances · 2023Article
- Regulation of Helicobacter pylori Urease and Acetone Carboxylase Genes by Nitric Oxide and the CrdRS Two-Component System.Microbiology spectrum · 2023Article
- Insights into the Orchestration of Gene Transcription Regulators inInternational journal of molecular sciences · 2022Review
- Delineation of the pH-Responsive Regulon Controlled by the Helicobacter pylori ArsRS Two-Component System.Infection and immunity · 2021Article
- Acid-responsive activity of theProceedings of the National Academy of Sciences of the United States of America · 2018Article
- Measurement of Internal pH in Helicobacter pylori by Using Green Fluorescent Protein Fluorimetry.Journal of bacteriology · 2018Article
- Acid-regulated gene expression of Helicobacter pylori: Insight into acid protection and gastric colonization.Helicobacter · 2018Article
- High-Salt Conditions Alter Transcription of Helicobacter pylori Genes Encoding Outer Membrane Proteins.Infection and immunity · 2018Article
- Phosphorylation-dependent and Phosphorylation-independent Regulation of Helicobacter pylori Acid Acclimation by the ArsRS Two-component System.Helicobacter · 2016Article
- Crosstalk between the HpArsRS two-component system and HpNikR is necessary for maximal activation of urease transcription.Frontiers in microbiology · 2015Article
- Factors that mediate colonization of the human stomach by Helicobacter pylori.World journal of gastroenterology · 2014Review
- A second-generation protein-protein interaction network of Helicobacter pylori.Molecular & cellular proteomics : MCP · 2014Article
- Themes and Variations: Regulation of RpoN-Dependent Flagellar Genes across Diverse Bacterial Species.Scientifica · 2014Review
- Role of the Helicobacter pylori sensor kinase ArsS in protein trafficking and acid acclimation.Journal of bacteriology · 2012Article
- Ni(II) coordination to mixed sites modulates DNA binding of HpNikR via a long-range effect.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Metal site occupancy and allosteric switching in bacterial metal sensor proteins.Archives of biochemistry and biophysics · 2012Review
- Acid-shock of Campylobacter jejuni induces flagellar gene expression and host cell invasion.European journal of microbiology & immunology · 2012Article
- Gastric infection by Helicobacter pylori.Current gastroenterology reports · 2011Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Helicobacter pylori colonizes the acidic gastric environment, in contrast to all other neutralophiles, whose acid resistance and tolerance responses allow only gastric transit. This acid adaptation is dependent on regulation of gene expression in response to pH changes in the periplasm and cytoplasm. The cytoplasmic histidine kinase, HP0244, which until now was thought only to regulate flagellar gene expression via its cognate response regulator, HP0703, was found to generate a response to declining medium pH. Although not required for survival at pH 4.5, HP0244 is required for survival at pH 2.5 with 10 mM urea after 30 min. Transcriptional profiling of a HP0244 deletion mutant grown at pH 7.4 confirmed the contribution of HP0244 to sigma(54) activation via HP0703 to coordinate flagellar biosynthesis by a pH-independent regulon that includes 14 flagellar genes. Microarray analysis of cells grown at pH 4.5 without urea revealed an additional 22 genes, including 4 acid acclimation genes (ureA, ureB, ureI, and amiE) that are positively regulated by HP0244. Additionally, 86 differentially expressed genes, including 3 acid acclimation genes (ureF, rocF [arginase], and ansB [asparaginase]), were found in cells grown at pH 2.5 with 30 mM urea. Hence, HP0244 has, in addition to the pH-independent flagellar regulon, a pH-dependent regulon, which allows adaptation to a wider range of environmental acid conditions. An acid survival study using an HP0703 mutant and an electrophoretic mobility shift assay with in vitro-phosphorylated HP0703 showed that HP0703 does not contribute to acid survival and does not bind to the promoter regions of several genes in the HP0244 pH-dependent regulon, suggesting that there is a pathway outside the HP0703 regulon which transduces the acid-responsive signal sensed by HP0244.
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Registered trials
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