ArticleVirology2009
Reverse genetic manipulation of the overlapping coding regions for structural proteins of the type II porcine reproductive and respiratory syndrome virus.
Article in Virology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- The commercial PRRSV attenuated vaccine can be a potentially effective live trivalent vaccine vector.Applied microbiology and biotechnology · 2025Article
- Porcine Reproductive and Respiratory Syndrome Virus Reverse Genetics and the Major Applications.Viruses · 2020Review
- Article
- Quasispecies evolution of the prototypical genotype 1 porcine reproductive and respiratory syndrome virus early during in vivo infection is rapid and tissue specific.Archives of virology · 2017Article
- Engineering the PRRS virus genome: updates and perspectives.Veterinary microbiology · 2014Review
- Development of porcine respiratory and reproductive syndrome virus replicon vector for foot-and-mouth disease vaccine.Clinical and experimental vaccine research · 2014Article
- Article
- Use of reverse genetics to develop a novel marker porcine reproductive and respiratory syndrome virus.Virus genes · 2012Article
- Arterivirus minor envelope proteins are a major determinant of viral tropism in cell culture.Journal of virology · 2012Article
- Cis-acting structural element in 5' UTR is essential for infectivity of porcine reproductive and respiratory syndrome virus.Virus research · 2012Article
- Identification of non-essential regions in nucleocapsid protein of porcine reproductive and respiratory syndrome virus for replication in cell culture.Virus research · 2011Article
- A 5'-proximal stem-loop structure of 5' untranslated region of porcine reproductive and respiratory syndrome virus genome is key for virus replication.Virology journal · 2011Article
- Identification of dispensable nucleotide sequence in 3' untranslated region of porcine reproductive and respiratory syndrome virus.Virus research · 2010Review
- Recombinant PRRSV expressing porcine circovirus sequence reveals novel aspect of transcriptional control of porcine arterivirus.Virus research · 2010Article
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The overlapping genomic regions coding for structural proteins of porcine reproductive and respiratory syndrome virus (PRRSV) poses problems for molecular dissection of the virus replication process. We constructed five mutant full-length cDNA clones with the overlapping regions unwound and 1 to 3 restriction sites inserted between two adjacent ORFs (ORF1/2, ORF4/5, ORF5/6, ORF 6/7 and ORF7/3' UTR), which generated the recombinant viruses. Our findings demonstrated that 1) the overlapping structural protein ORFs can be physically separated, and is dispensable for virus viability; 2) such ORF separations did not interrupt the subgenomic RNA synthesis; 3) the plaque morphology, growth kinetics, and antigenicity of these mutant viruses were virtually indistinguishable from those of the parental virus in cultured cells; and 4) these mutant viruses remained genetic stable in vitro. This study lays a foundation for further molecular dissection of PRRSV replication process, and development of genetically tagged vaccines against PRRS.
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