ArticlePlanta2009
Microspore embryogenesis in barley: anther pre-treatment stimulates plant defence gene expression.
Article in Planta, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
25 citing papers in PubMed, 46 citations in OpenAlex.
- From pollen precursor to totipotent embryo: a model system for plant cell developmental plasticity.Plant cell reports · 2026Review
- 5-azacytidine affects triticale microspore embryogenesis and is associated with modulation of reactive oxygen species, cysteine protease profiles, and programmed cell death.BMC plant biology · 2026Article
- Transcriptomic analyses reveal regulatory plasticity and metabolic reprogramming underlying genotype-specific microspore embryogenesis in wheat.Plant cell reports · 2026Article
- Molecular Insights into Microspore Embryogenesis: Transcriptomic Dynamics in Barley and Wheat.Methods in molecular biology (Clifton, N.J.) · 2026Review
- Adapting Crops to Rising Temperatures: Understanding Heat Stress and Plant Resilience Mechanisms.International journal of molecular sciences · 2025Review
- Modulation of calcium, callose synthesis, membrane permeability and pectin methyl-esterase activity affect cell wall composition and embryo yield during Brassica napus microspore embryogenesis.Annals of botany · 2025Article
- Transcriptomic identification of potential antioxidative enzyme regulators of the gametophytic-to-embryogenic switch in barley microspores.Frontiers in plant science · 2025Article
- New Prospects for Improving Microspore Embryogenesis Induction in Highly Recalcitrant Winter Wheat Lines.Plants (Basel, Switzerland) · 2024Article
- Impact of Ascorbate-Glutathione Cycle Components on the Effectiveness of Embryogenesis Induction in Isolated Microspore Cultures of Barley and Triticale.Antioxidants (Basel, Switzerland) · 2021Article
- Embryogenic competence of microspores is associated with their ability to form a callosic, osmoprotective subintinal layer.Journal of experimental botany · 2019Article
- Glutathione provides antioxidative defence and promotes microspore-derived embryo development in isolated microspore cultures of triticale (× Triticosecale Wittm.).Plant cell reports · 2019Article
- Differential Expression Profiling of Microspores During the Early Stages of Isolated Microspore Culture Using the Responsive Barley Cultivar Gobernadora.G3 (Bethesda, Md.) · 2018Article
- Review
- Analysis of wheat microspore embryogenesis induction by transcriptome and small RNA sequencing using the highly responsive cultivar "Svilena".BMC plant biology · 2016Article
- Pollen-Associated Microbiome Correlates with Pollution Parameters and the Allergenicity of Pollen.PloS one · 2016Article
- Current insights into hormonal regulation of microspore embryogenesis.Frontiers in plant science · 2015Review
- Alterations in the transcriptome of soybean in response to enhanced somatic embryogenesis promoted by orthologs of Agamous-like15 and Agamous-like18.Plant physiology · 2014Article
- Analysis of expression profiles of selected genes associated with the regenerative property and the receptivity to gene transfer during somatic embryogenesis in Triticum aestivum L.Molecular biology reports · 2013Article
- Article
- Insights into the regenerative property of plant cells and their receptivity to transgenesis: wheat as a research case study.Plant signaling & behavior · 2012Review
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microspore embryogenesis (ME) is a process in which the gametophytic pollen programme of the microspore is reoriented towards a new embryo sporophytic programme. This process requires a stress treatment, usually performed in the anther or isolated microspores for several days. Despite the universal use of stress to induce ME, very few studies have addressed the physiological processes that occur in the anther during this step. To further understand the processes triggered by stress treatment, we followed the response of anthers by measuring the expression of stress-related genes in two barley (Hordeum vulgare L.) cultivars differing in their ME response. Genes encoding enzymes involved in oxidative stress (glutathione-S-transferase, GST; oxalate oxidase, OxO), in the synthesis of jasmonic acid (13-lipoxygenase, Lox; allene oxide cyclase, AOC; allene oxide synthase, AOS) and in the phenylpropanoid pathway (phenylalanine ammonia lyase, PAL), as well as those encoding PR proteins (Barwin, chitinase 2b, Chit 2b; glucanase, Gluc; basic pathogenesis-related protein 1, PR1; pathogenesis-related protein 10, PR10) were up-regulated in whole anthers upon stress treatment, indicating that anther perceives stress and reacts by triggering general plant defence mechanisms. In particular, both OxO and Chit 2b genes are good markers of anther reactivity owing to their high level of induction during the stress treatment. The effect of copper sulphate appeared to limit the expression of defence-related genes, which may be correlated with its positive effect on the yield of microspore embryos.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.