ArticleJournal of fluorescence2009
Nucleobase-specific enhancement of Cy3 fluorescence.
Article in Journal of fluorescence, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed.
- Single-Molecule Chemistry Part II: Pathway Analysis of the Oxidation of Guanine to 8-Oxo-7,8-dihydroguanosine in an Oligonucleotide Hybrid.Molecules (Basel, Switzerland) · 2026Article
- Time-Resolved Fluorescence Detection of Nicked DNA via Site-Specific Stacking of Sulfo-Cy3: The Role of Charge, Polarity, Linker, and Sequence Context.The journal of physical chemistry. B · 2025Article
- Photostability of organic fluorophore influenced by adjacent amino acid residues.Communications chemistry · 2025Article
- A new twist on PIFE: photoisomerisation-related fluorescence enhancement.Methods and applications in fluorescence · 2023Review
- Article
- Electronic Structure and Excited-State Dynamics of DNA-Templated Monomers and Aggregates of Asymmetric Polymethine Dyes.The journal of physical chemistry. A · 2023Article
- Sequence-dependence of Cy3 and Cy5 dyes in 3' terminally-labeled single-stranded DNA.Scientific reports · 2022Article
- Targetable Conformationally Restricted Cyanines Enable Photon-Count-Limited Applications*.Angewandte Chemie (International ed. in English) · 2021Article
- Dependence of Fluorescence Quenching of CY3 Oligonucleotide Conjugates on the Oxidation Potential of the Stacking Base Pair.Molecules (Basel, Switzerland) · 2020Article
- Fit-free analysis of fluorescence lifetime imaging data using the phasor approach.Nature protocols · 2018Article
- A Rhodium-Cyanine Fluorescent Probe: Detection and Signaling of Mismatches in DNA.Journal of the American Chemical Society · 2017Article
- Directional Photonic Wire Mediated by Homo-Förster Resonance Energy Transfer on a DNA Origami Platform.ACS nano · 2017Article
- Sequence-Dependent Fluorescence of Cy3- and Cy5-Labeled Double-Stranded DNA.Bioconjugate chemistry · 2016Article
- Chemical modifications of artificial restriction DNA cutter (ARCUT) to promote its in vivo and in vitro applications.Artificial DNA, PNA & XNA · 2014Review
- A 1536-well fluorescence polarization assay to screen for modulators of the MUSASHI family of RNA-binding proteins.Combinatorial chemistry & high throughput screening · 2014Article
- Comparison of the sequence-dependent fluorescence of the cyanine dyes Cy3, Cy5, DyLight DY547 and DyLight DY647 on single-stranded DNA.PloS one · 2014Article
- A highly fluorescent DNA toolkit: synthesis and properties of oligonucleotides containing new Cy3, Cy5 and Cy3B monomers.Nucleic acids research · 2012Article
- Photophysics of fluorescent probes for single-molecule biophysics and super-resolution imaging.Annual review of physical chemistry · 2012Review
- Orientation of cyanine fluorophores terminally attached to DNA via long, flexible tethers.Biophysical journal · 2011Article
- Cy3-DNA stacking interactions strongly depend on the identity of the terminal basepair.Biophysical journal · 2011Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We report on the role of dye-nucleobase interactions on the photophysical properties of the indocarbocyanine Cy3. The fluorescence efficiency and lifetime ofCy3 increase in the presence of all four nucleoside monophosphates. This behavior correlates with an increase in the activation energy for photoisomerization and a approximately 4 nm red shift in the fluorescence spectrum. Changes are more dramatic for the purines (dAMP, dGMP) than the pyrimidines(dCMP, dTMP), and for the nonsulfonated cyanine(DiIC2(3)) than the sulfonated dye (Cy3-SE). These results are consistent with a model in which Cy3-nucleoside pi-pi interactions decrease the efficiency of photoisomerization,increasing the efficiency of fluorescence.
Indexed as
Identifiers
18972191What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.