ArticlePlant physiology2009
Suppression of 4-coumarate-CoA ligase in the coniferous gymnosperm Pinus radiata.
Article in Plant physiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
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Who cites it
59 citing papers in PubMed, 183 citations in OpenAlex.
- A Scots pine 4-coumarate:CoA ligase with high cinnamic acid affinity is the primary candidate for pinosylvin biosynthesis.The Plant journal : for cell and molecular biology · 2026Article
- GePIF4 Increases the Multi-Flower/Capsule-Bearing Traits and Gastrodin Biosynthesis inPlants (Basel, Switzerland) · 2025Article
- Pearl millet a promising fodder crop for changing climate: a review.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024Review
- Silencing ScGUX2 reduces xylan glucuronidation and improves biomass saccharification in sugarcane.Plant biotechnology journal · 2024Article
- Inducible Pluripotent Suspension Cell Cultures (iPSCs) to Study Plant Cell Differentiation.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Multiomics studies with co-transformation reveal microRNAs via miRNA-TF-mRNA network participating in wood formation inFrontiers in plant science · 2023Article
- Lignin engineering in forest trees: From gene discovery to field trials.Plant communications · 2022Review
- Review
- Thermophilic microbial deconstruction and conversion of natural and transgenic lignocellulose.Environmental microbiology reports · 2021Review
- Overexpression of vesicle-associated membrane protein PttVAP27-17 as a tool to improve biomass production and the overall saccharification yields in Populus trees.Biotechnology for biofuels · 2021Article
- Review
- Structural insight of two 4-Coumarate CoA ligase (3 Biotech · 2020Article
- Autofluorescence in Plants.Molecules (Basel, Switzerland) · 2020Review
- Article
- Lignin Engineering in Forest Trees.Frontiers in plant science · 2019Review
- Engineering abiotic stress response in plants for biomass production.The Journal of biological chemistry · 2018Review
- Cloning and Functional Characterization of Two 4-Coumarate: CoA Ligase Genes from Selaginella moellendorffii.Molecules (Basel, Switzerland) · 2018Article
- Lignins: Biosynthesis and Biological Functions in Plants.International journal of molecular sciences · 2018Review
- A multi-omics approach reveals function of Secretory Carrier-Associated Membrane Proteins in wood formation of Populus trees.BMC genomics · 2018Article
- Strategies for Engineering Reproductive Sterility in Plantation Forests.Frontiers in plant science · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Severe suppression of 4-coumarate-coenzyme A ligase (4CL) in the coniferous gymnosperm Pinus radiata substantially affected plant phenotype and resulted in dwarfed plants with a "bonsai tree-like" appearance. Microscopic analyses of stem sections from 2-year-old plants revealed substantial morphological changes in both wood and bark tissues. This included the formation of weakly lignified tracheids that displayed signs of collapse and the development of circumferential bands of axial parenchyma. Acetyl bromide-soluble lignin assays and proton nuclear magnetic resonance studies revealed lignin reductions of 36% to 50% in the most severely affected transgenic plants. Two-dimensional nuclear magnetic resonance and pyrolysis-gas chromatography-mass spectrometry studies indicated that lignin reductions were mainly due to depletion of guaiacyl but not p-hydroxyphenyl lignin. 4CL silencing also caused modifications in the lignin interunit linkage distribution, including elevated beta-aryl ether (beta-O-4 unit) and spirodienone (beta-1) levels, accompanied by lower phenylcoumaran (beta-5), resinol (beta-beta), and dibenzodioxocin (5-5/beta-O-4) levels. A sharp depletion in the level of saturated (dihydroconiferyl alcohol) end groups was also observed. Severe suppression of 4CL also affected carbohydrate metabolism. Most obvious was an up to approximately 2-fold increase in galactose content in wood from transgenic plants due to increased compression wood formation. The molecular, anatomical, and analytical data verified that the isolated 4CL clone is associated with lignin biosynthesis and illustrated that 4CL silencing leads to complex, often surprising, physiological and morphological changes in P. radiata.
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Registered trials
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