ArticleAmerican journal of physiology. Regulatory, integrative and comparative physiology2009
Heme oxygenase is necessary for the excitatory response of cultured neonatal rat rostral ventrolateral medulla neurons to hypoxia.
Article in American journal of physiology. Regulatory, integrative and comparative physiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 31 citations in OpenAlex.
- The Heme Oxygenase/Biliverdin Reductase System and Its Genetic Variants in Physiology and Diseases.Antioxidants (Basel, Switzerland) · 2025Review
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- Advances in cellular and integrative control of oxygen homeostasis within the central nervous system.The Journal of physiology · 2018Review
- Article
- Review
- On the existence of a central respiratory oxygen sensor.Journal of applied physiology (Bethesda, Md. : 1985) · 2017Review
- Eupnea, tachypnea, and autoresuscitation in a closed-loop respiratory control model.Journal of neurophysiology · 2017Article
- Time Domains of the Hypoxic Ventilatory Response and Their Molecular Basis.Comprehensive Physiology · 2016Review
- Functional Oxygen Sensitivity of Astrocytes.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2015Article
- A review on hemeoxygenase-2: focus on cellular protection and oxygen response.Oxidative medicine and cellular longevity · 2014Review
- A new method to detect rapid oxygen changes around cells: how quickly do calcium channels sense oxygen in cardiomyocytes?Journal of applied physiology (Bethesda, Md. : 1985) · 2013Article
- Hypoxic regulation of cardiac Ca2+ channel: possible role of haem oxygenase.The Journal of physiology · 2012Article
- Molecular mechanisms of chronic intermittent hypoxia and hypertension.Critical reviews in biomedical engineering · 2012Review
- Adenosine A₂a receptors and O₂ sensing in development.American journal of physiology. Regulatory, integrative and comparative physiology · 2011Review
- Elevated vertebrobasilar artery resistance in neonatal spontaneously hypertensive rats.Journal of applied physiology (Bethesda, Md. : 1985) · 2011Article
- Decay accelerating factor (CD55) protects neuronal cells from chemical hypoxia-induced injury.Journal of neuroinflammation · 2010Article
- Heme oxygenase is necessary for the excitatory response of cultured neonatal rat rostral ventrolateral medulla neurons to hypoxia by D'Agostino D, Mazza E, and Neubauer JA.American journal of physiology. Regulatory, integrative and comparative physiology · 2009Article
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3 authors at 1 institution in 1 country.
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Abstract
Heme oxygenase has been linked to the oxygen-sensing function of the carotid body, pulmonary vasculature, cerebral vasculature, and airway smooth muscle. We have shown previously that the cardiorespiratory regions of the rostral ventrolateral medulla are excited by local hypoxia and that heme oxygenase-2 (HO-2) is expressed in the hypoxia-chemosensitive regions of the rostral ventrolateral medulla (RVLM), the respiratory pre-Bötzinger complex, and C1 sympathoexcitatory region. To determine whether heme oxygenase is necessary for the hypoxic-excitation of dissociated RVLM neurons (P1) cultured on confluent medullary astrocytes (P5), we examined their electrophysiological responses to hypoxia (NaCN and low Po(2)) using the whole-cell perforated patch clamp technique before and after blocking heme oxygenase with tin protoporphyrin-IX (SnPP-IX). Following the electrophysiological recording, immunocytochemistry was performed on the recorded neuron to correlate the electrophysiological response to hypoxia with the expression of HO-2. We found that the responses to NaCN and hypoxia were similar. RVLM neurons responded to NaCN and low Po(2) with either depolarization or hyperpolarization and SnPP-IX blocked the depolarization response of hypoxia-excited neurons to both NaCN and low Po(2) but had no effect on the hyperpolarization response of hypoxia-depressed neurons. Consistent with this observation, HO-2 expression was present only in the hypoxia-excited neurons. We conclude that RVLM neurons are excited by hypoxia via a heme oxygenase-dependent mechanism.
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