ArticleProceedings of the National Academy of Sciences of the United States of America2008
Visualizing myosin-actin interaction with a genetically-encoded fluorescent strain sensor.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- Molecular Spies in Action: Genetically Encoded Fluorescent Biosensors Light up Cellular Signals.Chemical reviews · 2024Review
- Myosin II tension sensors visualize force generation within the actin cytoskeleton in living cells.Journal of cell science · 2024Article
- Molecular Force Sensors for Biological Application.International journal of molecular sciences · 2024Review
- Opticool: Cutting-edge transgenic optical tools.PLoS genetics · 2024Review
- Bioengineering tools for probing intracellular events in T lymphocytes.WIREs mechanisms of disease · 2021Review
- Magnetic probe-based microrheology reveals local softening and stiffening of 3D collagen matrices by fibroblasts.Biomedical microdevices · 2021Article
- Enhanced Molecular Tension Sensor Based on Bioluminescence Resonance Energy Transfer (BRET).ACS sensors · 2020Article
- Circularly Permuted Fluorescent Protein-Based Indicators: History, Principles, and Classification.International journal of molecular sciences · 2019Review
- Genetically Encoded Fluorescent Biosensors Illuminate the Spatiotemporal Regulation of Signaling Networks.Chemical reviews · 2018Review
- Quantifying forces in cell biology.Nature cell biology · 2017Review
- Design and development of genetically encoded fluorescent sensors to monitor intracellular chemical and physical parameters.Biophysical reviews · 2016Review
- Spider Silk Peptide Is a Compact, Linear Nanospring Ideal for Intracellular Tension Sensing.Nano letters · 2016Article
- Lighting Up the Force: Investigating Mechanisms of Mechanotransduction Using Fluorescent Tension Probes.Molecular and cellular biology · 2015Review
- Integrated micro/nanoengineered functional biomaterials for cell mechanics and mechanobiology: a materials perspective.Advanced materials (Deerfield Beach, Fla.) · 2014Review
- The use of nanoscale fluorescence microscopic to decipher cell wall modifications during fungal penetration.Frontiers in plant science · 2014Article
- Interrogating biology with force: single molecule high-resolution measurements with optical tweezers.Biophysical journal · 2013Review
- Force probing of individual molecules inside the living cell is now a reality.Nature chemical biology · 2012Review
- Orientation-based FRET sensor for real-time imaging of cellular forces.Journal of cell science · 2012Article
- Visualizing mechanical tension across membrane receptors with a fluorescent sensor.Nature methods · 2011Article
- Genetically encoded force sensors for measuring mechanical forces in proteins.Communicative & integrative biology · 2011Article
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Many proteins have been shown to undergo conformational changes in response to externally applied force in vitro, but whether the force-induced protein conformational changes occur in vivo remains unclear. To reveal the force-induced conformational changes, or strains, within proteins in living cells, we have developed a genetically encoded fluorescent "strain sensor," by combining the proximity imaging (PRIM) technique, which uses spectral changes of 2 GFP molecules that are in direct contact, and myosin-actin as a model system. The developed PRIM-based strain sensor module (PriSSM) consists of the tandem fusion of a normal and circularly permuted GFP. To apply strain to PriSSM, it was inserted between 2 motor domains of Dictyostelium myosin II. In the absence of strain, the 2 GFP moieties in PriSSM are in contact, whereas when the motor domains are bound to F-actin, PriSSM has a strained conformation, leading to the loss of contact and a concomitant spectral change. Using the sensor system, we found that the position of the lever arm in the rigor state was affected by mutations within the motor domain. Moreover, the sensor was used to visualize the interaction between myosin II and F-actin in Dictyostelium cells. In normal cells, myosin was largely detached from F-actin, whereas ATP depletion or hyperosmotic stress increased the fraction of myosin bound to F-actin. The PRIM-based strain sensor may provide a general approach for studying force-induced protein conformational changes in cells.
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