ArticleRNA (New York, N.Y.)2008
The role of the putative 3' end processing endonuclease Ysh1p in mRNA and snoRNA synthesis.
Article in RNA (New York, N.Y.), 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 27 citations in OpenAlex.
- Many paths to destruction: Family-specific turnover and stress responses for tRNA introns.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- MANY PATHS TO DESTRUCTION: FAMILY-SPECIFIC TURNOVER AND STRESS RESPONSES FOR TRNA INTRONS.bioRxiv : the preprint server for biology · 2025Article
- Advances in the mechanism of small nucleolar RNA and its role in DNA damage response.Military Medical Research · 2024Review
- Genetic and pharmacological evidence for kinetic competition between alternative poly(A) sites in yeast.eLife · 2021Article
- FttA is a CPSF73 homologue that terminates transcription in Archaea.Nature microbiology · 2020Article
- The APT complex is involved in non-coding RNA transcription and is distinct from CPF.Nucleic acids research · 2018Article
- A Defective mRNA Cleavage and Polyadenylation Complex Facilitates Expansions of Transcribed (GAA)Cell reports · 2017Article
- Transcription Dynamics in Living Cells.Annual review of biophysics · 2016Review
- Tunable and reversible drug control of protein production via a self-excising degron.Nature chemical biology · 2015Article
- Pcf11 orchestrates transcription termination pathways in yeast.Genes & development · 2015Article
- A unified model for yeast transcript definition.Genome research · 2014Article
- DNA damage induces targeted, genome-wide variation of poly(A) sites in budding yeast.Genome research · 2013Article
- A quantitative, high-throughput reverse genetic screen reveals novel connections between Pre-mRNA splicing and 5' and 3' end transcript determinants.PLoS genetics · 2012Article
- Unravelling the means to an end: RNA polymerase II transcription termination.Nature reviews. Molecular cell biology · 2011Review
- The P-loop domain of yeast Clp1 mediates interactions between CF IA and CPF factors in pre-mRNA 3' end formation.PloS one · 2011Article
- Novel endoribonucleases as central players in various pathways of eukaryotic RNA metabolism.RNA (New York, N.Y.) · 2010Review
- Distinct roles of non-canonical poly(A) polymerases in RNA metabolism.PLoS genetics · 2009Article
- Transcription termination by nuclear RNA polymerases.Genes & development · 2009Article
- Cordycepin interferes with 3' end formation in yeast independently of its potential to terminate RNA chain elongation.RNA (New York, N.Y.) · 2009Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pre-mRNA 3' end formation is tightly linked to upstream and downstream events of eukaryotic mRNA synthesis. The two-step reaction involves endonucleolytic cleavage of the primary transcript followed by poly(A) addition to the upstream cleavage product. To further characterize the putative 3' end processing endonuclease Ysh1p/Brr5p, we isolated and analyzed a number of new temperature- and cold-sensitive mutant alleles. We show that Ysh1p plays a crucial role in 3' end formation and in RNA polymerase II (RNAP II) transcription termination on mRNA genes. In addition, we observed a range of additional functional deficiencies in ysh1 mutant strains, which were partially allele-specific. Interestingly, snoRNA 3' end formation and RNAP II termination were defective on specific snoRNAs in the cold-sensitive ysh1-12 strain. Moreover, we observed the accumulation of several mRNAs including the NRD1 transcript in this mutant. We provide evidence that NRD1 autoregulation is associated with endonucleolytic cleavage and that this process may involve Ysh1p. In addition, the ysh1-12 strain displayed defects in RNA splicing indicating that a functional link may exist between intron removal and 3' end formation in yeast. These observations suggest that Ysh1p has multiple roles in RNA synthesis and processing.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.