ArticleRNA (New York, N.Y.)2008
Structure of yeast U6 snRNPs: arrangement of Prp24p and the LSm complex as revealed by electron microscopy.
Article in RNA (New York, N.Y.), 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
15 citing papers in PubMed, 31 citations in OpenAlex.
- Terminal nucleotidyl transferases (TENTs) in mammalian RNA metabolism.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2018Review
- Architecture of the U6 snRNP reveals specific recognition of 3'-end processed U6 snRNA.Nature communications · 2018Article
- The sole LSm complex inRNA (New York, N.Y.) · 2017Article
- Core structure of the U6 small nuclear ribonucleoprotein at 1.7-Å resolution.Nature structural & molecular biology · 2014Article
- Structural analyses of the pre-mRNA splicing machinery.Protein science : a publication of the Protein Society · 2013Review
- Lsm proteins and Hfq: Life at the 3' end.RNA biology · 2013Review
- Whole brain and brain regional coexpression network interactions associated with predisposition to alcohol consumption.PloS one · 2013Article
- A novel occluded RNA recognition motif in Prp24 unwinds the U6 RNA internal stem loop.Nucleic acids research · 2011Article
- Spliceosome structure and function.Cold Spring Harbor perspectives in biology · 2011Review
- The spliceosomal proteome: at the heart of the largest cellular ribonucleoprotein machine.Proteomics · 2010Review
- Ribonucleoprotein multimers and their functions.Critical reviews in biochemistry and molecular biology · 2010Review
- Merging molecular electron microscopy and mass spectrometry by carbon film-assisted endoproteinase digestion.Molecular & cellular proteomics : MCP · 2010Article
- Structure and functional implications of a complex containing a segment of U6 RNA bound by a domain of Prp24.RNA (New York, N.Y.) · 2010Article
- (1)H, (13)C and (15)N resonance assignments of a ribonucleoprotein complex consisting of Prp24-RRM2 bound to a fragment of U6 RNA.Biomolecular NMR assignments · 2009Article
- 3' processing of eukaryotic precursor tRNAs.Wiley interdisciplinary reviews. RNAReview
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Protein components of the U6 snRNP (Prp24p and LSm2-8) are thought to act cooperatively in facilitating the annealing of U6 and U4 snRNAs during U4/U6 di-snRNP formation. To learn more about the spatial arrangement of these proteins in S. cerevisiae U6 snRNPs, we investigated the structure of this particle by electron microscopy. U6 snRNPs, purified by affinity chromatography and gradient centrifugation, and then immediately adsorbed to the carbon film support, revealed an open form in which the Prp24 protein and the ring formed by the LSm proteins were visible as two separate morphological domains, while particles stabilized by chemical cross-linking in solution under mild conditions before binding to the carbon film exhibited a compact form, with the two domains in close proximity to one another. In the open form, individual LSm proteins were located by a novel approach employing C-terminal genetic tagging of the LSm proteins with yECitrine. These studies show the Prp24 protein at defined distances from each subunit of the LSm ring, which in turn suggests that the LSm ring is positioned in a consistent manner on the U6 RNA. Furthermore, in agreement with the EM observations, UV cross-linking revealed U6 RNA in contact with the LSm2 protein at the interface between Prp24p and the LSm ring. Further, LSmp-Prp24p interactions may be restricted to the closed form, which appears to represent the solution structure of the U6 snRNP particle.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.