ArticleMolecular vision2008
Abnormal epithelial homeostasis in the cornea of mice with a destrin deletion.
Article in Molecular vision, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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Who cites it
20 citing papers in PubMed, 29 citations in OpenAlex.
- Plasticity of ocular surface epithelia: Using a murine model of limbal stem cell deficiency to delineate metaplasia and transdifferentiation.Stem cell reports · 2022Article
- Zebrafish cornea formation and homeostasis reveal a slow maturation process, similarly to terrestrial vertebrates' corneas.Frontiers in physiology · 2022Article
- RNA nanoparticle distribution and clearance in the eye after subconjunctival injection with and without thermosensitive hydrogels.Journal of controlled release : official journal of the Controlled Release Society · 2018Article
- Inhibition of TGF-β signaling supports high proliferative potential of diverse p63Scientific reports · 2017Article
- Comparison of two related lines of tauGFP transgenic mice designed for lineage tracing.BMC developmental biology · 2017Article
- Vortex Pattern of Corneal Deposits in Granular Corneal Dystrophy Associated With the p.(Arg555Trp) Mutation in TGFBI.Cornea · 2017Article
- Intracellular Position of Centrioles and the Direction of Homeostatic Epithelial Cell Movements in the Mouse Cornea.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2017Article
- The core planar cell polarity gene,Royal Society open science · 2016Article
- Abnormal corneal epithelial maintenance in mice heterozygous for the micropinna microphthalmia mutation Mp.Experimental eye research · 2016Article
- Lineage tracing in the adult mouse corneal epithelium supports the limbal epithelial stem cell hypothesis with intermittent periods of stem cell quiescence.Stem cell research · 2015Article
- Evaluating alternative stem cell hypotheses for adult corneal epithelial maintenance.World journal of stem cells · 2015Review
- Stem Cells in the Cornea.Progress in molecular biology and translational science · 2015Review
- Serum response factor: positive and negative regulation of an epithelial gene expression network in the destrin mutant cornea.Physiological genomics · 2014Article
- Changes in ADF/destrin expression in the development of hair cells following Atoh1-induced ectopic regeneration.Experimental and therapeutic medicine · 2013Article
- Increased corneal epithelial turnover contributes to abnormal homeostasis in the Pax6(+/-) mouse model of aniridia.PloS one · 2013Article
- Normal X-inactivation mosaicism in corneas of heterozygous FlnaDilp2/+ female mice--a model of human filamin A (FLNA) diseases.BMC research notes · 2012Article
- Stem cells and corneal epithelial maintenance: insights from the mouse and other animal models.Results and problems in cell differentiation · 2012Review
- Interaction between hedgehog signalling and PAX6 dosage mediates maintenance and regeneration of the corneal epithelium.Molecular vision · 2012Article
- Effects of aberrant Pax6 gene dosage on mouse corneal pathophysiology and corneal epithelial homeostasis.PloS one · 2011Article
- A pathogenic relationship between a regulator of the actin cytoskeleton and serum response factor.Genetics · 2010Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
purposeDstn(corn1) mice lack normal destrin expression and develop corneal abnormality shortly after birth such as epithelial hyperplasia and total vascularization. Thus, the mice serve as a model for ocular surface disorders. To determine the nature of epithelial defects, we examined whether epithelial homeostasis is altered in these corneas.
methodsDstn(corn1) mice were crossed with ubiquitous GFP mice to generate a double homozygous line, GFP-Dstn(corn1), and cell movements were determined by whole-mount histology and in vivo time-lapse microscopy, tracking the change of epithelial GFP patterns. Rates of cell division and the presence of label-retaining cells (LRCs) were determined by systemic bromodeoxyuridine (BrdU). Epithelial expression of keratins 8, 12, and 15, and MUC5AC were determined by whole-mount immunofluorescence.
resultsEpithelial cells in an adult GFP-Dstn(corn1) cornea were generally immobile with no sign of directed movement for the entire life of the animal. These cells were not senescent because more than 70% of basal epithelial cells incorporated BrdU over a 24 h period. LRCs were widely distributed throughout a GFP-Dstn(corn1) cornea. The epithelium of a GFP-Dstn(corn1) cornea contained a mixed population of cells with a corneal and a conjunctival phenotype as judged by the expression of keratins and MUC5AC.
conclusionsEpithelial cells of an adult GFP-Dstn(corn1) cornea are generally stationary, mitotically active, and contain LRCs, indicating that the epithelium is self-sustained, which in turn suggests that epithelial stem cells are present within the cornea. Epithelial homeostasis of adult GFP-Dstn(corn1) corneas is abnormal, mimicking that of a normal conjunctiva or a pathological, conjunctivalized cornea.
Indexed as
Identifiers
18958303PMC2573731W2167307690What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.