ArticleGenetics2009
Stringent mating-type-regulated auxotrophy increases the accuracy of systematic genetic interaction screens with Saccharomyces cerevisiae mutant arrays.
Article in Genetics, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 22 citations in OpenAlex.
- QTL mapping, breeding, and debugging Saccharomyces cerevisiae strains through Reiterated Mass Selection and backcrosSing (ReMaSSing).Biotechnology for biofuels and bioproducts · 2026Article
- A synthetic genetic array screen for interactions with the RNA helicase DED1 during cell stress in budding yeast.G3 (Bethesda, Md.) · 2023Article
- Genetic interactions derived from high-throughput phenotyping of 6589 yeast cell cycle mutants.NPJ systems biology and applications · 2020Article
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- Yeast Phenomics: An Experimental Approach for Modeling Gene Interaction Networks that Buffer Disease.Genes · 2015Article
- Conserved allosteric hot spots in the transmembrane domains of cystic fibrosis transmembrane conductance regulator (CFTR) channels and multidrug resistance protein (MRP) pumps.The Journal of biological chemistry · 2014Article
- Phenomic assessment of genetic buffering by kinetic analysis of cell arrays.Methods in molecular biology (Clifton, N.J.) · 2014Article
- The SWI/SNF chromatin remodeling complex influences transcription by RNA polymerase I in Saccharomyces cerevisiae.PloS one · 2013Article
- A yeast phenomic model for the gene interaction network modulating CFTR-ΔF508 protein biogenesis.Genome medicine · 2012Article
- Bioinformatic identification of genes suppressing genome instability.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Recruitment of Rpd3 to the telomere depends on the protein arginine methyltransferase Hmt1.PloS one · 2012Article
- Recursive expectation-maximization clustering: a method for identifying buffering mechanisms composed of phenomic modules.Chaos (Woodbury, N.Y.) · 2010Article
- Genomewide analysis reveals novel pathways affecting endoplasmic reticulum homeostasis, protein modification and quality control.Genetics · 2009Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
A genomic collection of haploid Saccharomyces cerevisiae deletion strains provides a unique resource for systematic analysis of gene interactions. Double-mutant haploid strains can be constructed by the synthetic genetic array (SGA) method, wherein a query mutation is introduced by mating to mutant arrays, selection of diploid double mutants, induction of meiosis, and selection of recombinant haploid double-mutant progeny. The mechanism of haploid selection is mating-type-regulated auxotrophy (MRA), by which prototrophy is restricted to a particular haploid genotype generated only as a result of meiosis. MRA escape leads to false-negative genetic interaction results because postmeiotic haploids that are supposed to be under negative selection instead proliferate and mate, forming diploids that are heterozygous at interacting loci, masking phenotypes that would be observed in a pure haploid double-mutant culture. This work identified factors that reduce MRA escape, including insertion of terminator and repressor sequences upstream of the MRA cassette, deletion of silent mating-type loci, and utilization of alpha-type instead of a-type MRA. Modifications engineered to reduce haploid MRA escape reduced false negative results in SGA-type analysis, resulting in >95% sensitivity for detecting gene-gene interactions.
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