ArticleJournal of cell science2008
Ncd motor binding and transport in the spindle.
Article in Journal of cell science, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- The Clinical Significance of KIFs in Cervical and Endometrial Cancer: from Mechanisms to Biomarker and Therapeutic Potential.Reproductive sciences (Thousand Oaks, Calif.) · 2026Review
- Mechanical states of a motor protein in the spindle.Current biology : CB · 2026Article
- What Determines the Directionality of Kinesins? Studying the Anomalous Directionality of Nonclaret Disjunctional Motor Mutants.Journal of the American Chemical Society · 2026Article
- Asymmetry of centrosomes inMolecular biology of the cell · 2025Article
- Role of motor proteins in human cancers.Saudi journal of biological sciences · 2022Review
- Analysis of chemomechanical behavior of stress fibers by continuum mechanics-based FRAP.Biophysical journal · 2022Article
- Article
- Klp2 and Ase1 synergize to maintain meiotic spindle stability during metaphase I.The Journal of biological chemistry · 2020Article
- RanGTP induces an effector gradient of XCTK2 and importin α/β for spindle microtubule cross-linking.The Journal of cell biology · 2020Article
- Diffusive tail anchorage determines velocity and force produced by kinesin-14 between crosslinked microtubules.Nature communications · 2018Article
- Review
- Interplay between microtubule bundling and sorting factors ensures acentriolar spindle stability during C. elegans oocyte meiosis.PLoS genetics · 2017Article
- Anastral spindle assembly and γ-tubulin in Drosophila oocytes.BMC cell biology · 2011Article
- Kinesins at a glance.Journal of cell science · 2010Article
- Mitotic force generators and chromosome segregation.Cellular and molecular life sciences : CMLS · 2010Review
- Prometaphase spindle maintenance by an antagonistic motor-dependent force balance made robust by a disassembling lamin-B envelope.The Journal of cell biology · 2010Article
- Opposite-polarity motors activate one another to trigger cargo transport in live cells.The Journal of cell biology · 2009Article
- Mature Drosophila meiosis I spindles comprise microtubules of mixed polarity.Current biology : CB · 2009Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
The Ncd kinesin-14 motor is required for meiotic spindle assembly in Drosophila oocytes and produces force in mitotic spindles that opposes other motors. Despite extensive studies, the way the motor binds to the spindle to perform its functions is not well understood. By analyzing Ncd deleted for the conserved head or the positively charged tail, we found that the tail is essential for binding to spindles and centrosomes, but both the head and tail are needed for normal spindle assembly and function. Fluorescence photobleaching assays to analyze binding interactions with the spindle yielded data for headless and full-length Ncd that did not fit well to previous recovery models. We report a new model that accounts for Ncd transport towards the equator revealed by fluorescence flow analysis of early mitotic spindles and gives rate constants that confirm the dominant role the Ncd tail plays in binding to the spindle. By contrast, the head binds weakly to spindles based on analysis of the tailless fluorescence recovery data. Minus-end Ncd thus binds tightly to spindles and is transported in early metaphase towards microtubule plus-ends, the opposite direction to that in which the motor moves, to produce force in the spindle later in mitosis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.