ArticleThe Journal of biological chemistry2009
Analysis of histones in Xenopus laevis. I. A distinct index of enriched variants and modifications exists in each cell type and is remodeled during developmental transitions.
Article in The Journal of biological chemistry, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
44 citing papers in PubMed.
- Genomic Insights into Fertilization: Tracing PLCZ1 Orthologs Across Amphibian Lineages.Genome biology and evolution · 2025Article
- On the Hunt for the Histone Code.Molecular & cellular proteomics : MCP · 2024Article
- Genomic disturbance of vitellogenin 2 (vtg2) leads to vitellin membrane deficiencies and significant mortalities at early stages of embryonic development in zebrafish (Danio rerio).Scientific reports · 2023Article
- Article
- A rapidly evolving single copy histone H1 variant is associated with male fertility in a parasitoid wasp.Frontiers in cell and developmental biology · 2023Article
- Article
- The tumour suppressor brain tumour (Brat) regulates linker histone dBigH1 expression in theOpen biology · 2021Article
- Metabolic and Epigenetics Action Mechanisms of Antiobesity Medicinal Plants and Phytochemicals.Evidence-based complementary and alternative medicine : eCAM · 2021Review
- Epigenetic homogeneity in histone methylation underlies sperm programming for embryonic transcription.Nature communications · 2020Article
- Long-term association of a transcription factor with its chromatin binding site can stabilize gene expression and cell fate commitment.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Genome wide decrease of DNA replication eye density at the midblastula transition ofCell cycle (Georgetown, Tex.) · 2019Article
- Reconstituting Nuclear and Chromosome Dynamics UsingCold Spring Harbor protocols · 2019Article
- Temporally and Spatially Regulated Expression of the Linker Histone H1fx During Mouse Development.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2017Article
- Article
- Article
- Germline-specific H1 variants: the "sexy" linker histones.Chromosoma · 2016Review
- Chromatin assembly and transcriptional cross-talk in Xenopus laevis oocyte and egg extracts.The International journal of developmental biology · 2016Article
- The cnidarian Hydractinia echinata employs canonical and highly adapted histones to pack its DNA.Epigenetics & chromatin · 2016Article
- Chaperone-mediated chromatin assembly and transcriptional regulation in Xenopus laevis.The International journal of developmental biology · 2016Review
- The PRMT5 arginine methyltransferase: many roles in development, cancer and beyond.Cellular and molecular life sciences : CMLS · 2015Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
Abstract
Histone proteins contain epigenetic information that is encoded both in the relative abundance of core histones and variants and particularly in the post-translational modification of these proteins. We determined the presence of such variants and covalent modifications in seven tissue types of the anuran Xenopus laevis, including oocyte, egg, sperm, early embryo equivalent (pronuclei incubated in egg extract), S3 neurula cells, A6 kidney cells, and erythrocytes. We first developed a new robust method for isolating the stored, predeposition histones from oocytes and eggs via chromatography on heparin-Sepharose, whereas we isolated chromatinized histones via conventional acid extraction. We identified two previously unknown H1 isoforms (H1fx and H1B.Sp) present on sperm chromatin. We immunoblotted this global collection of histones with many specific post-translational modification antibodies, including antibodies against methylated histone H3 on Lys(4), Lys(9), Lys(27), Lys(79), Arg(2), Arg(17), and Arg(26); methylated histone H4 on Lys(20); methylated H2A and H4 on Arg(3); acetylated H4 on Lys(5), Lys(8), Lys(12), and Lys(16) and H3 on Lys(9) and Lys(14); and phosphorylated H3 on Ser(10) and H2A/H4 on Ser(1). Furthermore, we subjected a subset of these histones to two-dimensional gel analysis and subsequent immunoblotting and mass spectrometry to determine the global remodeling of histone modifications that occurs as development proceeds. Overall, our observations suggest that each metazoan cell type may have a unique histone modification signature correlated with its differentiation status.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.