ArticleThe Journal of biological chemistry2008
Chloride Is essential for capacitation and for the capacitation-associated increase in tyrosine phosphorylation.
Article in The Journal of biological chemistry, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed.
- Chloride Intracellular Channel 4 (CLIC4) controls volume regulation in sperm development via Protein Kinase C (PKC).BMC biology · 2026Article
- TMEM16A Maintains Acrosomal Integrity Through ERK1/2, RhoA, and Actin Cytoskeleton During Capacitation.International journal of molecular sciences · 2025Article
- Attenuation of Ampullary Anoctamin 1 by the peritoneal fluid in rhesus macaques with spontaneous endometriosis†.Biology of reproduction · 2025Article
- Aqp4a and Trpv4 mediate regulatory cell volume increase for swimming maintenance of marine fish spermatozoa.Cellular and molecular life sciences : CMLS · 2024Article
- Review
- Membrane potential hyperpolarization: a critical factor in acrosomal exocytosis and fertilization in sperm within the female reproductive tract.Frontiers in cell and developmental biology · 2024Article
- The Ubiquitin-Proteasome System Participates in Sperm Surface Subproteome Remodeling during Boar Sperm Capacitation.Biomolecules · 2023Article
- Morphological and Molecular Bases of Male Infertility: A Closer Look at Sperm Flagellum.Genes · 2023Review
- A Review on the Role of Bicarbonate and Proton Transporters during Sperm Capacitation in Mammals.International journal of molecular sciences · 2022Review
- Article
- pH-dependent effects of procaine on equine gamete activation†.Biology of reproduction · 2019Article
- CFTR/ENaC-dependent regulation of membrane potential during human sperm capacitation is initiated by bicarbonate uptake through NBC.The Journal of biological chemistry · 2018Article
- Molecular Basis of Human Sperm Capacitation.Frontiers in cell and developmental biology · 2018Review
- Essential Role of CFTR in PKA-Dependent Phosphorylation, Alkalinization, and Hyperpolarization During Human Sperm Capacitation.Journal of cellular physiology · 2017Article
- OSR1 and SPAK cooperatively modulate Sertoli cell support of mouse spermatogenesis.Scientific reports · 2016Article
- The tyrosine kinase FER is responsible for the capacitation-associated increase in tyrosine phosphorylation in murine sperm.Development (Cambridge, England) · 2016Article
- Biphasic role of calcium in mouse sperm capacitation signaling pathways.Journal of cellular physiology · 2015Article
- Central role of soluble adenylyl cyclase and cAMP in sperm physiology.Biochimica et biophysica acta · 2014Review
- The pattern of tyrosine phosphorylation in human sperm in response to binding to zona pellucida or hyaluronic acid.Reproductive sciences (Thousand Oaks, Calif.) · 2014Article
- Article
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Authors and funding
8 authors.
Funding
Abstract
After epididymal maturation, sperm capacitation, which encompasses a complex series of molecular events, endows the sperm with the ability to fertilize an egg. This process can be mimicked in vitro in defined media, the composition of which is based on the electrolyte concentration of the oviductal fluid. It is well established that capacitation requires Na(+), HCO(3)(-), Ca(2+), and a cholesterol acceptor; however, little is known about the function of Cl(-) during this important process. To determine whether Cl(-), in addition to maintaining osmolarity, actively participates in signaling pathways that regulate capacitation, Cl(-) was replaced by either methanesulfonate or gluconate two nonpermeable anions. The absence of Cl(-) did not affect sperm viability, but capacitation-associated processes such as the increase in tyrosine phosphorylation, the increase in cAMP levels, hyperactivation, the zona pellucidae-induced acrosome reaction, and most importantly, fertilization were abolished or significantly reduced. Interestingly, the addition of cyclic AMP agonists to sperm incubated in Cl(-)-free medium rescued the increase in tyrosine phosphorylation and hyperactivation suggesting that Cl(-) acts upstream of the cAMP/protein kinase A signaling pathway. To investigate Cl(-) transport, sperm incubated in complete capacitation medium were exposed to a battery of anion transport inhibitors. Among them, bumetanide and furosemide, two blockers of Na(+)/K(+)/Cl(-) cotransporters (NKCC), inhibited all capacitation-associated events, suggesting that these transporters may mediate Cl(-) movements in sperm. Consistent with these results, Western blots using anti-NKCC1 antibodies showed the presence of this cotransporter in mature sperm.
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