ArticleXenotransplantation
Proteomic identification of non-Gal antibody targets after pig-to-primate cardiac xenotransplantation.
Article in Xenotransplantation. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed, 95 citations in OpenAlex.
- Xeno kidney: revolutionizing kidney disease treatment.Frontiers in nephrology · 2026Review
- T cell-mediated immune responses in xenotransplantation: mechanisms and therapeutic strategies.Frontiers in immunology · 2026Review
- 2025: status of cardiac xenotransplantation including preclinical models.Frontiers in transplantation · 2025Review
- Pig Lung Xenotransplantation: Barriers on the Road to Clinical Translation.Transplant international : official journal of the European Society for Organ Transplantation · 2025Review
- Graft rejection in paediatric congenital heart disease.Translational pediatrics · 2023Review
- Progress in xenotransplantation: overcoming immune barriers.Nature reviews. Nephrology · 2022Review
- Engineering Efforts to Refine Compatibility and Duration of Aortic Valve Replacements: An Overview of Previous Expectations and New Promises.Frontiers in cardiovascular medicine · 2022Review
- Cardiac Xenotransplantation: Progress in Preclinical Models and Prospects for Clinical Translation.Transplant international : official journal of the European Society for Organ Transplantation · 2022Review
- Current status of xenotransplantation research and the strategies for preventing xenograft rejection.Frontiers in immunology · 2022Review
- Proteomics Analysis of Aqueous Humor and Rejected Graft in Pig-to-Non-Human Primate Corneal Xenotransplantation.Frontiers in immunology · 2022Article
- The Possible Role of Anti-Neu5Gc as an Obstacle in Xenotransplantation.Frontiers in immunology · 2020Review
- Xenotransplantation: Current Status in Preclinical Research.Frontiers in immunology · 2019Review
- B4GALNT2 and xenotransplantation: A newly appreciated xenogeneic antigen.Xenotransplantation · 2018Review
- The impact of serum incubation time on IgM/IgG binding to porcine aortic endothelial cells.Xenotransplantation · 2017Article
- Antigenicity of Bovine Pericardium Determined by a Novel Immunoproteomic Approach.Scientific reports · 2017Article
- Cardiac Non-Human Leukocyte Antigen Identification: Techniques and Troubles.Frontiers in immunology · 2017Review
- Lung xenotransplantation: a review.Current opinion in organ transplantation · 2016Review
- Interspecies chimeric complementation for the generation of functional human tissues and organs in large animal hosts.Transgenic research · 2016Review
- The production of multi-transgenic pigs: update and perspectives for xenotransplantation.Transgenic research · 2016Review
- Recent investigations into pig antigen and anti-pig antibody expression.International journal of surgery (London, England) · 2015Review
Corrections and comments
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Authors and funding
13 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundExperience with non-antigenic galactose alpha1,3 galactose (alphaGal) polymers and development of alphaGal deficient pigs has reduced or eliminated the significance of this antigen in xenograft rejection. Despite these advances, delayed xenograft rejection (DXR) continues to occur most likely due to antibody responses to non-Gal endothelial cell (EC) antigens.
methodsTo gauge the diversity of the non-Gal antibody response we used antibody derived from CD46 transgenic heterotopic cardiac xenografts performed without T-cell immunosuppression, Group A (n = 4) and Gal knockout (GT-KO) heart transplants under tacrolimus and sirolimus immunosuppression, Group B (n = 8). Non-Gal antibody was measured by flow cytometry and by western blots using GT-KO EC membrane antigens. A nanoLC/MS/MS analysis of proteins recovered from 2D gels was used to identify target antigens.
resultsGroup A recipients exhibited a mixed cellular and humoral rejection. Group B recipients mainly exhibited classical DXR. Western blot analysis showed a non-Gal antibody response induced by GT+ and GT-KO hearts to an overlapping set of pig aortic EC membrane antigens. Proteomic analysis identified 14 potential target antigens but failed to define several immunodominant targets.
conclusionsThese experiments indicate that the non-Gal antibody response is directed to a number of stress response and inflammation related pig EC antigens and a few undefined targets. Further analysis of these antibody specificities using alternative methods is required to more fully define the repertoire of non-Gal antibody responses.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.