ArticleThe Journal of experimental medicine2008
A novel genetic strategy reveals unexpected roles of the Swi-Snf-like chromatin-remodeling BAF complex in thymocyte development.
Article in The Journal of experimental medicine, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
21 citing papers in PubMed, 33 citations in OpenAlex.
- Targeting RIPK1 kinase for modulating inflammation in human diseases.Frontiers in immunology · 2023Review
- DDIT4 S-Nitrosylation Aids p38-MAPK Signaling Complex Assembly to Promote Hepatic Reactive Oxygen Species Production.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Article
- BET bromodomain protein inhibition reverses chimeric antigen receptor extinction and reinvigorates exhausted T cells in chronic lymphocytic leukemia.The Journal of clinical investigation · 2021Article
- COMPASS and SWI/SNF complexes in development and disease.Nature reviews. Genetics · 2021Review
- In situ conversion of defective Treg into SuperTreg cells to treat advanced IPEX-like disorders in mice.Nature communications · 2020Article
- Article
- The Loss of TET2 Promotes CD8Journal of immunology (Baltimore, Md. : 1950) · 2018Article
- BPTF Is Essential for T Cell Homeostasis and Function.Journal of immunology (Baltimore, Md. : 1950) · 2016Article
- ATP-dependent chromatin remodeling during mammalian development.Development (Cambridge, England) · 2016Review
- BRG1-mediated immune tolerance: facilitation of Treg activation and partial independence of chromatin remodelling.The EMBO journal · 2013Article
- Combined gene dosage requirement for SWI/SNF catalytic subunits during early mammalian development.Mammalian genome : official journal of the International Mammalian Genome Society · 2013Article
- A general approach for controlling transcription and probing epigenetic mechanisms: application to the CD4 locus.Journal of immunology (Baltimore, Md. : 1950) · 2013Article
- LoxP-FRT Trap (LOFT): a simple and flexible system for conventional and reversible gene targeting.BMC biology · 2012Article
- Essential roles of the chromatin remodeling factor BRG1 in spermatogenesis in mice.Biology of reproduction · 2012Article
- ATP-dependent chromatin remodeling in T cells.Biochemistry and cell biology = Biochimie et biologie cellulaire · 2012Review
- The nucleosome remodeling factor.FEBS letters · 2011Review
- Regulation of nucleosome landscape and transcription factor targeting at tissue-specific enhancers by BRG1.Genome research · 2011Article
- NF-κB and BRG1 bind a distal regulatory element in the IL-3/GM-CSF locus.Molecular immunology · 2011Article
- Varying modulation of HIV-1 LTR activity by Baf complexes.Journal of molecular biology · 2011Article
- Dynamic BRG1 recruitment during T helper differentiation and activation reveals distal regulatory elements.Molecular and cellular biology · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 3 institutions in 1 country.
Funding
Abstract
We have developed a general strategy for creating littermates bearing either a tissue-specific point mutation or deletion in any target gene, and used the method to dissect the roles of Brg, the ATPase subunit of the chromatin-remodeling Brg-associated factor (BAF) complex, in early thymocyte development. We found that a point mutation that inactivates the Brg ATPase recapitulates multiple defects previously described for Brg deletion (Chi, T.H., M. Wan, P.P. Lee, K. Akashi, D. Metzger, P. Chambon, C.B. Wilson, and G.R. Crabtree. 2003. Immunity. 19:169-182). However, the point mutant helps reveal unexpected roles of Brg in CD25 repression and CD4 activation. Surprisingly, CD4 activation occurs independently of the Brg ATPase and is perhaps mediated by physical interactions between Brg and the CD4 locus. Our study thus suggests that the BAF complex harbors novel activities that can be necessary and even sufficient for stimulating transcription from an endogenous chromatin template in the absence of Brg-dependent remodeling of that template. We conclude that conditional point mutants, rarely used in mammalian genetics, can help uncover important gene functions undetectable or overlooked in deletion mutants.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.