ArticleAntimicrobial agents and chemotherapy2009
Novel chimeric beta-lactamase CTX-M-64, a hybrid of CTX-M-15-like and CTX-M-14 beta-lactamases, found in a Shigella sonnei strain resistant to various oxyimino-cephalosporins, including ceftazidime.
Article in Antimicrobial agents and chemotherapy, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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26 citing papers in PubMed, 51 citations in OpenAlex.
- Prevalence of antimicrobial-resistantOne health (Amsterdam, Netherlands) · 2026Article
- Cross-sectoral sharing of CTX-M-producingMicrobiology spectrum · 2026Article
- Characterizations ofFrontiers in microbiology · 2023Article
- Fecal Carriage and Genetic Characterization of CTX-M-1/9/1-ProducingFrontiers in microbiology · 2021Article
- Structural Comparisons of Cefotaximase (CTX-M-ase) Sub Family 1.Frontiers in microbiology · 2021Review
- Wastewater as a Probable Environmental Reservoir of Extended-Spectrum-β-Lactamase Genes: Detection of Chimeric β-Lactamases CTX-M-64 and CTX-M-123.Applied and environmental microbiology · 2019Article
- Article
- Characterization of Protein Domain Function viaBio-protocol · 2018Article
- Functional Characterization of CTX-M-14 and CTX-M-15 β-Lactamases byAntimicrobial agents and chemotherapy · 2017Article
- Bacterium-Derived Cell-Penetrating Peptides Deliver Gentamicin To Kill Intracellular Pathogens.Antimicrobial agents and chemotherapy · 2017Article
- Direct identification of antibiotic resistance genes on single plasmid molecules using CRISPR/Cas9 in combination with optical DNA mapping.Scientific reports · 2016Article
- Comparative Characterization of CTX-M-64 and CTX-M-14 Provides Insights into the Structure and Catalytic Activity of the CTX-M Class of Enzymes.Antimicrobial agents and chemotherapy · 2016Article
- Molecular characterization and analysis of high-level multidrug-resistance of Shigella flexneri serotype 4s strains from China.Scientific reports · 2016Article
- Residues Distal to the Active Site Contribute to Enhanced Catalytic Activity of Variant and Hybrid β-Lactamases Derived from CTX-M-14 and CTX-M-15.Antimicrobial agents and chemotherapy · 2015Article
- blaCTX-M-1/9/1 Hybrid Genes May Have Been Generated from blaCTX-M-15 on an IncI2 Plasmid.Antimicrobial agents and chemotherapy · 2015Article
- Detection of CTX-M-64 in Escherichia coli isolates from human patients in China.Antimicrobial agents and chemotherapy · 2015Article
- Genomic portrait of the evolution and epidemic spread of a recently emerged multidrug-resistant Shigella flexneri clone in China.Journal of clinical microbiology · 2014Article
- Characterization of extended-spectrum beta-lactamase-producing clinical isolates of Shigella flexneri.Journal of health, population, and nutrition · 2013Article
- CTX-M-123, a novel hybrid of the CTX-M-1 and CTX-M-9 Group β-lactamases recovered from Escherichia coli isolates in China.Antimicrobial agents and chemotherapy · 2013Article
- Generation of the blaCTX-M-116 hybrid gene.Antimicrobial agents and chemotherapy · 2013Article
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Authors and funding
5 authors at 3 institutions in 1 country.
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Abstract
The plasmid-mediated novel beta-lactamase CTX-M-64 was first identified in Shigella sonnei strain UIH-1, which exhibited resistance to cefotaxime (MIC, 1,024 microg/ml) and ceftazidime (MIC, 32 microg/ml). The amino acid sequence of CTX-M-64 showed a chimeric structure of a CTX-M-15-like beta-lactamase (N- and C-terminal moieties) and a CTX-M-14-like beta-lactamase (central portion, amino acids 63 to 226), suggesting that it originated by homologous recombination between the corresponding genes. The introduction of a recombinant plasmid carrying bla(CTX-M-64) conferred resistance to cefotaxime in Escherichia coli, and the activities of cefotaxime and ceftazidime were restored in the presence of clavulanic acid. Of note, CTX-M-64 production could also confer consistent resistance to ceftazidime, which differs from the majority of CTX-M-type enzymes, which poorly hydrolyze ceftazidime. These results were consistent with the kinetic parameters determined with the purified CTX-M-64 enzyme. The bla(CTX-M-64) gene was flanked upstream by an ISEcp1 sequence and downstream by an orf477 sequence. The sequence of the 45-bp spacer region between the right inverted repeat (IRR) of ISEcp1 and bla(CTX-M-64) was exactly identical to that of ISEcp1-bla(CTX-M-15-like). Moreover, the presence of a putative IRR of ISEcp1 at the right end of truncated orf477 is indicative of an ISEcp1-mediated transposition event in the bla(CTX-M-64) gene. The emergence of CTX-M-64 by probable homologous recombination would suggest the natural potential of an alternative mechanism for the diversification of CTX-M-type beta-lactamases.
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