ArticleMolecular and cellular biology2009
Binding of Drosophila ORC proteins to anaphase chromosomes requires cessation of mitotic cyclin-dependent kinase activity.
Article in Molecular and cellular biology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 37 citations in OpenAlex.
- A mitotic bookmark coordinates transcription and replication in Drosophila embryos.Nucleic acids research · 2025Article
- The origin recognition complex requires chromatin tethering by a hypervariable intrinsically disordered region that is functionally conserved from sponge to man.Nucleic acids research · 2024Article
- Molecular determinants of phase separation foreLife · 2021Article
- Tissue-Specific DNA Replication Defects inGenetics · 2020Article
- A new class of disordered elements controls DNA replication through initiator self-assembly.eLife · 2019Article
- Spatial and temporal resolution of mORC4 fluorescent variants reveals structural requirements for achieving higher order self-association and pronuclei entry.Methods and applications in fluorescence · 2019Article
- Rapid embryonic cell cycles defer the establishment of heterochromatin by Eggless/SetDB1 inGenes & development · 2019Article
- Article
- Genome Duplication: The Heartbeat of Developing Organisms.Current topics in developmental biology · 2016Review
- Drosophila Rif1 is an essential gene and controls late developmental events by direct interaction with PP1-87B.Scientific reports · 2015Article
- Orc1 Binding to Mitotic Chromosomes Precedes Spatial Patterning during G1 Phase and Assembly of the Origin Recognition Complex in Human Cells.The Journal of biological chemistry · 2015Article
- Spatio-temporal regulation of the human licensing factor Cdc6 in replication and mitosis.Cell cycle (Georgetown, Tex.) · 2015Article
- Dynamic changes in the genomic localization of DNA replication-related element binding factor during the cell cycle.Cell cycle (Georgetown, Tex.) · 2013Article
- Unique pattern of ORC2 and MCM7 localization during DNA replication licensing in the mouse zygote.Biology of reproduction · 2012Article
- Phosphorylation of ORC2 protein dissociates origin recognition complex from chromatin and replication origins.The Journal of biological chemistry · 2012Article
- The dynamics of replication licensing in live Caenorhabditis elegans embryos.The Journal of cell biology · 2012Article
- "The Octet": Eight Protein Kinases that Control Mammalian DNA Replication.Frontiers in physiology · 2012Article
- The origin recognition complex: a biochemical and structural view.Sub-cellular biochemistry · 2012Review
- Preferential re-replication of Drosophila heterochromatin in the absence of geminin.PLoS genetics · 2010Article
- Recruitment of Orc6l, a dormant maternal mRNA in mouse oocytes, is essential for DNA replication in 1-cell embryos.Developmental biology · 2010Article
Corrections and comments
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2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The initial step in the acquisition of replication competence by eukaryotic chromosomes is the binding of the multisubunit origin recognition complex, ORC. We describe a transgenic Drosophila model which enables dynamic imaging of a green fluorescent protein (GFP)-tagged Drosophila melanogaster ORC subunit, DmOrc2-GFP. It is functional in genetic complementation, expressed at physiological levels, and participates quantitatively in complex formation. This fusion protein is therefore able to depict both the holocomplex DmOrc1-6 and the core complex DmOrc2-6 formed by the Drosophila initiator proteins. Its localization can be monitored in vivo along the cell cycle and development. DmOrc2-GFP is not detected on metaphase chromosomes but binds rapidly to anaphase chromatin in Drosophila embryos. Expression of either stable cyclin A, B, or B3 prevents this reassociation, suggesting that cessation of mitotic cyclin-dependent kinase activity is essential for binding of the DmOrc proteins to chromosomes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.