ArticleInfection and immunity2009
Genome-wide screen in Francisella novicida for genes required for pulmonary and systemic infection in mice.
Article in Infection and immunity, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
62 citing papers in PubMed, 83 citations in OpenAlex.
- Characterization of carABpyrB operon and role of pyrE in Francisella novicida biofilm.MicrobiologyOpen · 2026Article
- Development, Strategies, and Challenges for Tularemia Vaccine.Current microbiology · 2024Review
- Diverse roles of low-molecular weight thiol GSH inCurrent research in microbial sciences · 2024Article
- Atomic Structure of IglD Demonstrates Its Role as a Component of the Baseplate Complex of themBio · 2022Article
- ThioredoxinA1 Controls the Oxidative Stress Response of Francisella tularensis Live Vaccine Strain (LVS).Journal of bacteriology · 2022Article
- An AraC/XylS Family Transcriptional Regulator Modulates the Oxidative Stress Response of Francisella tularensis.Journal of bacteriology · 2021Article
- The pentose phosphate pathway constitutes a major metabolic hub in pathogenic Francisella.PLoS pathogens · 2021Article
- Type VI Secretion System and Its Effectors PdpC, PdpD, and OpiA Contribute toInfection and immunity · 2021Article
- Prokaryotic Solute/Sodium Symporters: Versatile Functions and Mechanisms of a Transporter Family.International journal of molecular sciences · 2021Review
- A study of innate immune kinetics reveals a role for a chloride transporter in a virulent Francisella tularensis type B strain.MicrobiologyOpen · 2021Article
- Genetic Determinants of Antibiotic Resistance inFrontiers in microbiology · 2021Review
- Tn-Seq reveals hidden complexity in the utilization of host-derived glutathione in Francisella tularensis.PLoS pathogens · 2020Article
- Deletion of glutaredoxin promotes oxidative tolerance and intracellular infection inVirulence · 2019Article
- Global Analysis of Genes Essential for Francisella tularensis Schu S4 GrowthJournal of bacteriology · 2019Article
- Thioredoxin-A is a virulence factor and mediator of the type IV pilus system in Acinetobacter baumannii.PloS one · 2019Article
- Transcription Elongation Factor GreA Plays a Key Role in Cellular Invasion and Virulence of Francisella tularensis subsp. novicida.Scientific reports · 2018Article
- Outer membrane vesicle-associated lipase FtlA enhances cellular invasion and virulence in Francisella tularensis LVS.Emerging microbes & infections · 2017Article
- Oxidative stress, protein damage and repair in bacteria.Nature reviews. Microbiology · 2017Review
- An Expanded Transposon Mutant Library Reveals that Vibrio fischeri δ-Aminolevulinate Auxotrophs Can Colonize Euprymna scolopes.Applied and environmental microbiology · 2017Article
- The Protease Locus of Francisella tularensis LVS Is Required for Stress Tolerance and Infection in the Mammalian Host.Infection and immunity · 2016Article
2 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 2 institutions in 1 country.
Funding
Abstract
Francisella tularensis is a gram-negative, highly infectious, aerosolizable facultative intracellular pathogen that causes the potentially life-threatening disease tularemia. To date there is no approved vaccine available, and little is known about the molecular mechanisms important for infection, survival, and dissemination at different times of infection. We report the first whole-genome screen using an inhalation mouse model to monitor infection in the lung and dissemination to the liver and spleen. We queried a comprehensive library of 2,998 sequence-defined transposon insertion mutants in Francisella novicida strain U112 using a microarray-based negative-selection screen. We were able to track the behavior of 1,029 annotated genes, equivalent to a detection rate of 75% and corresponding to approximately 57% of the entire F. novicida genome. As expected, most transposon mutants retained the ability to colonize, but 125 candidate virulence genes (12%) could not be detected in at least one of the three organs. They fell into a variety of functional categories, with one-third having no annotated function and a statistically significant enrichment of genes involved in transcription. Based on the observation that behavior during complex pool infections correlated with the degree of attenuation during single-strain infection we identified nine genes expected to strongly contribute to infection. These included two genes, those for ATP synthase C (FTN_1645) and thioredoxin (FTN_1415), that when mutated allowed increased host survival and conferred protection in vaccination experiments.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.