ArticleSexually transmitted infections2009
Herpes simplex virus type 2 antibody detection performance in Kisumu, Kenya, using the Herpeselect ELISA, Kalon ELISA, Western blot and inhibition testing.
Article in Sexually transmitted infections, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 1 of them a synthesis that pooled it.
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Who cites it
21 citing papers in PubMed, 1 synthesis or guideline pooled it, 41 citations in OpenAlex.
- 2024 European guidelines for the management of genital herpes.Journal of the European Academy of Dermatology and Venereology : JEADV · 2025Guideline
- Disclosure of HSV-2 serological test results in the context of an adolescent HIV prevention trial in Kenya.Sexually transmitted infections · 2015Trial
- Circumcision status and incident herpes simplex virus type 2 infection, genital ulcer disease, and HIV infection.AIDS (London, England) · 2012Trial
- Review
- Menstrual cups to reduce bacterial vaginosis and STIs through reduced harmful sexual and menstrual practices among economically vulnerable women: protocol of a single arm trial in western Kenya.BMC public health · 2024Article
- An Assessment of Risk Factors for Herpes Simplex Virus Type 2 Infection in Malawian Women Using 2 Classifications for the HerpeSelect 2 Test.Sexually transmitted diseases · 2020Article
- Computational sensing of herpes simplex virus using a cost-effective on-chip microscope.Scientific reports · 2017Article
- Performance of Kalon herpes simplex virus 2 assay using dried blood spots among young women in Uganda.African journal of laboratory medicine · 2016Article
- Precision of the Kalon Herpes Simplex Virus Type 2 IgG ELISA: an international inter-laboratory assessment.BMC infectious diseases · 2015Article
- Global Diversity within and between Human Herpesvirus 1 and 2 Glycoproteins.Journal of virology · 2015Article
- Prevalence and incidence estimation of HSV-2 by two IgG ELISA methods among South African women at high risk of HIV.PloS one · 2015Article
- Article
- Use of HIV and HSV-2 biomarkers in sub-saharan adolescent prevention research: a comparison of two approaches.The journal of primary prevention · 2014Article
- Incident HSV-2 infections are common among HIV-1-discordant couples.The Journal of infectious diseases · 2013Article
- Herpes simplex virus type-2 assay specificity and male circumcision to reduce herpes simplex virus type-2 acquisition.AIDS (London, England) · 2013Article
- Factors associated with herpes simplex virus type 2 incidence in a cohort of human immunodeficiency virus type 1-seronegative Kenyan men and women reporting high-risk sexual behavior.Sexually transmitted diseases · 2011Article
- Association between HSV1 seropositivity and obesity: data from the National Health and Nutritional Examination Survey, 2007-2008.PloS one · 2011Article
- Performance of the Focus HerpeSelect-2 enzyme immunoassay for the detection of herpes simplex virus type 2 antibodies in seven African countries.Sexually transmitted infections · 2011Article
- Sensitivity and specificity of herpes simplex virus-2 serological assays among HIV-infected and uninfected urban Ugandans.International journal of STD & AIDS · 2010Article
- Comparison of focus HerpesSelect and Kalon HSV-2 gG2 ELISA serological assays to detect herpes simplex virus type 2 antibodies in a South African population.Sexually transmitted infections · 2010Article
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Authors and funding
9 authors at 7 institutions in 3 countries.
Funding
Abstract
backgroundIn certain parts of Africa, type-specific herpes simplex virus type 2 (HSV-2) ELISAs may have limited specificity. To date, no study has been conducted to validate HerpeSelect and Kalon type-specific HSV-2 ELISAs using both the Western blot and recombinant gG ELISA inhibition testing as reference standards.
methodsA total of 120 men who were HIV seronegative (aged 18-24 years) provided blood samples. HSV-2 IgG serum antibodies were detected using four different methods: HerpeSelect HSV-2 ELISA (n = 120), Kalon HSV-2 ELISA (n = 120), University of Washington Western blot (n = 101) and a recombinant inhibition test (n = 93).
resultsHSV-2 seroprevalence differed significantly by HSV-2 detection method, ranging from 24.8% with the Western blot to 69.8% with the HerpeSelect ELISA. Using the Western blot as the reference standard, the HerpesSelect had the highest sensitivity for HSV-2 antibody detection (100%) yet lowest specificity (40%). Similar results were obtained using the inhibition test as the reference standard. The sensitivity and specificity of the Kalon test versus the Western blot were 92% and 79%, respectively, and 80% and 82% versus the inhibition test. Using the inhibition test as the reference standard, the sensitivity of the Western blot appeared low (49%).
conclusionsIn men in western Kenya who were HIV seronegative, the HerpeSelect and Kalon type-specific ELISAs had high sensitivities yet limited specificities using the Western blot as reference standard. Overall, the Kalon ELISA performed better than the HerpeSelect ELISA in these young men from Kisumu. Further understanding is needed for the interpretation of HSV-2 inhibition or ELISA test positive/ Western blot seronegative results. Before HSV-2 seropositivity may be reliably reported in selected areas of Africa, performance studies of HSV-2 serological assays in individual geographical areas are recommended.
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