Evidence map›Paper›PMID 18955382›Full record

Trial reportThe Journal of physiology2008

Immobilization induces anabolic resistance in human myofibrillar protein synthesis with low and high dose amino acid infusion.

Elisa I Glover, Stuart M Phillips, Bryan R Oates, Jason E Tang, Mark A Tarnopolsky, Anna Selby, Kenneth Smith, Michael J Rennie

Registry-linked trialOpen access · bronzeAbstract readClinical Trial
PubMed Full text at PMC Publisher
In one paragraph

Trial report in The Journal of physiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT07690150 (A Novel Angle), which is not on this map. Cited by 185 papers, 5 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
185citing papers in PubMed, 5 pooled it
7.4field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

NCT07690150 nanot yet recruitingnot on this mapstarted 2026, after this paper: background citation

A Novel Angle: Manipulating Knee Position to Understand Muscle Deterioration During Leg Immobilization

TypeinterventionalSponsorWageningen UniversityRan2026 to 2028Enrolled30ConditionsMuscle Atrophy, Disuse Atrophy, ImmobilizationArmsKnee immobilization brace (0°) for 5 days, Knee immobilization brace (60°) for 5 days
3 · Its place in the literature

Who cites it

185 citing papers in PubMed, 5 syntheses or guidelines pooled it, 405 citations in OpenAlex.

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125 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 2 countries.

Elisa I GloverDepartment of Kinesiology - Exercise Metabolism Research Group, McMaster University, 1280 Main St West, Hamilton, ON L8S 4K1, Canada.
Stuart M Phillips
Bryan R Oates
Jason E Tang
Mark A Tarnopolsky
Anna Selby
Kenneth Smith
Michael J Rennie
University of Nottingham · GBMcMaster University · CA

Funding

Biotechnology and Biological Sciences Research Council
6 · The paper itself

Abstract

We tested the hypothesis that increasing blood amino acid (AA) availability would counter the physical inactivity-induced reduction in muscle protein synthesis. We determined how 14 days of unilateral knee immobilization affected quadriceps myofibrillar protein synthesis (MPS) in young healthy subjects (10 men, 2 women, 21 +/- 1 years; 80.2 +/- 4.0 kg, mean +/- S.E.M.) in the post-absorptive state and after infusing AA (10% Primene) at low or high doses (43 and 261 mg kg(-1) h(-1)). Muscle cross-sectional area (MRI) and peak isometric torque declined in the immobilized leg (-5.0 +/- 1.2% and -25 +/- 3%, respectively, both P < 0.005), but were unchanged (all P > 0.6) in the non-immobilized leg. Immobilization induced a 27% decline in the rate of post-absorptive MPS (immobilized, 0.027 +/- 0.003: non-immobilized, 0.037 +/- 0.003% h(-1); P < 0.001). Regardless of dose, AA infusion stimulated a greater rise in MPS in the non-immobilized legs; at 4 h MPS was greater by +54 +/- 12% with low dose and +68 +/- 17% with high dose AA infusion (both P < 0.001). There was some evidence of delayed responsiveness of phosphorylation of Akt to high doses of AA and p70S6k at both doses but no marked differences in that of mTOR, GSK3beta or eEF2. Phosphorylation of focal adhesion kinase (Tyr(576/577)) was reduced (P < 0.05) with immobilization. We observed no change in polyubiquitinated protein content after immobilization. We confirm that 14 days of immobilization reduces MPS in the post-absorptive state and this diminution is reduced but not abolished by increased provision of AA, even at high rates. The immobilization-induced decline in post-absorptive MPS with the 'anabolic resistance' to amino acids can account for much of immobilization-induced muscle atrophy.

Indexed as

AdultAmino AcidsAmino Acids, EssentialDose-Response Relationship, DrugElongation Factor 2 KinaseFemaleFocal Adhesion Protein-Tyrosine KinasesGlycogen Synthase Kinase 3Glycogen Synthase Kinase 3 betaHumansImmobilizationInfusions, IntravenousInsulinMaleMuscle ProteinsMuscle StrengthAmino AcidsAmino Acids, EssentialElongation Factor 2 KinaseFocal Adhesion Protein-Tyrosine KinasesGlycogen Synthase Kinase 3Glycogen Synthase Kinase 3 betaGSK3B protein, humanInsulinMTOR protein, humanMuscle ProteinsProtein KinasesProto-Oncogene Proteins c-aktRibosomal Protein S6 Kinases, 70-kDaTOR Serine-Threonine Kinases

Identifiers

PMID18955382
PMCPMC2655417
OpenAlexW1573938781

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.