ArticleEndocrine2009
Ghrelin stimulates proliferation of human osteoblastic TE85 cells via NO/cGMP signaling pathway.
Article in Endocrine, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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Who cites it
11 citing papers in PubMed, 34 citations in OpenAlex.
- Energizing Healing with Electromagnetic Field Therapy in Musculoskeletal Disorders.Journal of orthopaedics and sports medicine · 2024Article
- Ghrelin uses the GHS-R1a/Gi/cAMP pathway and induces differentiation only in mature osteoblasts. This ghrelin pathway is impaired in AIS patients.Biochemistry and biophysics reports · 2020Article
- Zhuang-Gu-Fang Treats Osteoporosis in Ovariectomized Rats by Increasing the Osteogenesis-Related Factors Leptin, Ghrelin, and PYY.Evidence-based complementary and alternative medicine : eCAM · 2020Article
- Article
- Genetic determination of the cellular basis of the ghrelin-dependent bone remodeling.Molecular metabolism · 2015Article
- Cytokines and Hormones That Contribute to the Positive Association between Fat and Bone.Frontiers in endocrinology · 2014Review
- Electromagnetic fields act via activation of voltage-gated calcium channels to produce beneficial or adverse effects.Journal of cellular and molecular medicine · 2013Review
- Bone health and the female athlete triad in adolescent athletes.The Physician and sportsmedicine · 2011Review
- Relationship between gut and sepsis: Role of ghrelin.World journal of diabetes · 2011Article
- Nitric oxide inhibits ghrelin-induced cell proliferation and ERK1/2 activation in GH3 cells.Endocrine · 2010Article
- Expression and in vitro functions of the ghrelin axis in endometrial cancer.Hormones & cancer · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ghrelin regulates bone formation and osteoblast proliferation, but the detailed signaling pathway for its action on osteoblasts remains unclear. In human osteoblastic TE85 cells, we observed the effects and intracellular signaling pathway of ghrelin on cell proliferation using BrdU incorporation method. Ghrelin, at 10(-10)-10(-8) M concentration, significantly increased BrdU incorporation into TE85 cells. The action of ghrelin was inhibited by D: -Lys3-GHRP-6, a selective antagonist of GHS-R. Nitric oxide (NO) scavenger hemoglobin and the NO synthase inhibitor NAME eliminated the stimulatory action of ghrelin on proliferation, while NO donor SNAP and NO synthase substrate L-AME stimulated proliferation of osteoblastic TE85 cells. The cGMP analogue, 8-Br-cGMP, stimulated TE85 cell proliferation, and ghrelin did not enhance proliferation in the presence of 8-Br-cGMP. Inhibition of cGMP production by the guanylate cyclase inhibitor prevented ghrelin-induced osteoblastic TE85 cell proliferation. In conclusion, ghrelin stimulates proliferation of human osteoblastic TE85 cells via intracellular NO/cGMP signaling pathway.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.