ArticleEukaryotic cell2008
Localization and interaction of the proteins constituting the GAL genetic switch in Saccharomyces cerevisiae.
Article in Eukaryotic cell, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 27 citations in OpenAlex.
- Selective Modulation of Dynamic Protein Complexes.Cell chemical biology · 2020Review
- The pathway intermediate 2-keto-3-deoxy-L-galactonate mediates the induction of genes involved in D-galacturonic acid utilization in Aspergillus niger.FEBS letters · 2017Article
- Hsp90 Maintains Proteostasis of the Galactose Utilization Pathway To Prevent Cell Lethality.Molecular and cellular biology · 2016Article
- A cell size- and cell cycle-aware stochastic model for predicting time-dynamic gene network activity in individual cells.BMC systems biology · 2015Article
- Nucleo-cytoplasmic shuttling dynamics of the transcriptional regulators XYR1 and CRE1 under conditions of cellulase and xylanase gene expression in Trichoderma reesei.Molecular microbiology · 2014Article
- Regulations of sugar transporters: insights from yeast.Current genetics · 2013Review
- Molecular simulation and docking studies of Gal1p and Gal3p proteins in the presence and absence of ligands ATP and galactose: implication for transcriptional activation of GAL genes.Journal of computer-aided molecular design · 2012Article
- Interplay of a ligand sensor and an enzyme in controlling expression of the Saccharomyces cerevisiae GAL genes.Eukaryotic cell · 2012Article
- The Gal3p transducer of the GAL regulon interacts with the Gal80p repressor in its ligand-induced closed conformation.Genes & development · 2012Article
- Review
- Rapid GAL gene switch of Saccharomyces cerevisiae depends on nuclear Gal3, not nucleocytoplasmic trafficking of Gal3 and Gal80.Genetics · 2011Article
- Dynamic analysis of the KlGAL regulatory system in Kluyveromyces lactis: a comparative study with Saccharomyces cerevisiae.Systems and synthetic biology · 2011Article
- Impact of nonnatural amino acid mutagenesis on the in vivo function and binding modes of a transcriptional activator.Journal of the American Chemical Society · 2009Article
- Gene activation by dissociation of an inhibitor from a transcriptional activation domain.Molecular and cellular biology · 2009Article
- Role of chromatin states in transcriptional memory.Biochimica et biophysica acta · 2009Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
In Saccharomyces cerevisiae, the GAL genes encode the enzymes required for galactose metabolism. Regulation of these genes has served as the paradigm for eukaryotic transcriptional control over the last 50 years. The switch between inert and active gene expression is dependent upon three proteins--the transcriptional activator Gal4p, the inhibitor Gal80p, and the ligand sensor Gal3p. Here, we present a detailed spatial analysis of the three GAL regulatory proteins produced from their native genomic loci. Using a novel application of photobleaching, we demonstrate, for the first time, that the Gal3p ligand sensor enters the nucleus of yeast cells in the presence of galactose. Additionally, using Förster resonance energy transfer, we show that the interaction between Gal3p and Gal80p occurs throughout the yeast cell. Taken together, these data challenge existing models for the cellular localization of the regulatory proteins during the induction of GAL gene expression by galactose and suggest a mechanism for the induction of the GAL genes in which galactose-bound Gal3p moves from the cytoplasm to the nucleus to interact with the transcriptional inhibitor Gal80p.
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