ArticleApplied and environmental microbiology2008
Population heterogeneity of Lactobacillus plantarum WCFS1 microcolonies in response to and recovery from acid stress.
Article in Applied and environmental microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 57 citations in OpenAlex.
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- Gut microbe Lactiplantibacillus plantarum undergoes different evolutionary trajectories between insects and mammals.BMC biology · 2022Article
- Metabolic and Proteomic Profiles Reveal the Response of the ASD-Associated Resistant Strain 6-1 ofInternational journal of environmental research and public health · 2022Article
- Development of Resistance to Clarithromycin and Amoxicillin-Clavulanic Acid inMicrobiology spectrum · 2022Article
- Spatiotemporal bio-shielding of bacteria through consolidated geometrical structuring.NPJ biofilms and microbiomes · 2022Article
- The Application of Imaging Flow Cytometry for Characterisation and Quantification of Bacterial Phenotypes.Frontiers in cellular and infection microbiology · 2021Article
- Transient MutS-Based Hypermutation System for Adaptive Evolution of Lactobacillus casei to Low pH.Applied and environmental microbiology · 2017Article
- Quasi-continuous parallel online scattered light, fluorescence and dissolved oxygen tension measurement combined with monitoring of the oxygen transfer rate in each well of a shaken microtiter plate.Microbial cell factories · 2016Article
- Bacterial Colonies in Solid Media and Foods: A Review on Their Growth and Interactions with the Micro-Environment.Frontiers in microbiology · 2015Review
- Article
- Hyperosmotic stress response of Campylobacter jejuni.Journal of bacteriology · 2012Article
- Impact of respiration on resistance of Lactobacillus plantarum WCFS1 to acid stress.Applied and environmental microbiology · 2012Article
- Glutamine synthetase and glucose-6-phosphate isomerase are adhesive moonlighting proteins of Lactobacillus crispatus released by epithelial cathelicidin LL-37.Journal of bacteriology · 2012Article
- Article
- Short- and long-term adaptation to ethanol stress and its cross-protective consequences in Lactobacillus plantarum.Applied and environmental microbiology · 2011Article
- Single-cell genomics: unravelling the genomes of unculturable microorganisms.Microbial biotechnology · 2011Article
- The role of physiological heterogeneity in microbial population behavior.Nature chemical biology · 2010Review
- Analysis of Lactobacillus sakei mutants selected after adaptation to the gastrointestinal tracts of axenic mice.Applied and environmental microbiology · 2010Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Within an isogenic microbial population in a homogenous environment, individual bacteria can still exhibit differences in phenotype. Phenotypic heterogeneity can facilitate the survival of subpopulations under stress. As the gram-positive bacterium Lactobacillus plantarum grows, it acidifies the growth medium to a low pH. We have examined the growth of L. plantarum microcolonies after rapid pH downshift (pH 2 to 4), which prevents growth in liquid culture. This acidification was achieved by transferring cells from liquid broth onto a porous ceramic support, placed on a base of low-pH MRS medium solidified using Gelrite. We found a subpopulation of cells that displayed phenotypic heterogeneity and continued to grow at pH 3, which resulted in microcolonies dominated by viable but elongated (filamentous) cells lacking septation, as determined by scanning electron microscopy and staining cell membranes with the lipophilic dye FM4-64. Recovery of pH-stressed cells from these colonies was studied by inoculation onto MRS-Gelrite-covered slides at pH 6.5, and outgrowth was monitored by microscopy. The heterogeneity of the population, calculated from the microcolony areas, decreased with recovery from pH 3 over a period of a few hours. Filamentous cells did not have an advantage in outgrowth during recovery. Specific regions within single filamentous cells were more able to form rapidly dividing cells, i.e., there was heterogeneity even within single recovering cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.