ArticleApplied and environmental microbiology2008
Novel method for selection of antimicrobial peptides from a phage display library by use of bacterial magnetic particles.
Article in Applied and environmental microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Phage Display-Derived Peptides Have Neutralizing Activities Against Biofilm Formation byPharmaceuticals (Basel, Switzerland) · 2026Article
- Antimicrobial peptides: from discovery to developmental applications.Applied and environmental microbiology · 2025Review
- Phage Display Panning on Silica: Optimization of Elution Conditions for Selection of Strong Binders.Langmuir : the ACS journal of surfaces and colloids · 2024Article
- Investigation of Peptides for Molecular Recognition of C-Reactive Protein-Theoretical and Experimental Studies.Analytical chemistry · 2023Article
- Strategies and opportunities for engineering antifungal peptides for therapeutic applications.Current opinion in biotechnology · 2023Review
- Innovative Approaches of Engineering Tumor-Targeting Bacteria with Different Therapeutic Payloads to Fight Cancer: A Smart Strategy of Disease Management.International journal of nanomedicine · 2021Review
- Microbial Fabricated Nanosystems: Applications in Drug Delivery and Targeting.Frontiers in chemistry · 2021Review
- Article
- Magnetotactic bacteria as potential sources of bioproducts.Marine drugs · 2015Review
- Peptide design for antimicrobial and immunomodulatory applications.Biopolymers · 2013Review
- Development of anti-infectives using phage display: biological agents against bacteria, viruses, and parasites.Antimicrobial agents and chemotherapy · 2012Review
- Engineering building blocks for self-assembling protein nanoparticles.Microbial cell factories · 2010Article
Corrections and comments
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Authors and funding
3 authors.
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Abstract
Antimicrobial peptides were isolated from a phage display peptide library using bacterial magnetic particles (BacMPs) as a solid support. The BacMPs obtained from "Magnetospirillum magneticum" strain AMB-1 consist of pure magnetite (50 to 100 nm in size) and are covered with a lipid bilayer membrane derived from the invagination of the inner membrane. BacMPs are easily purified from a culture of magnetotactic bacteria by magnetic separation. Approximately 4 x 10(10) PFU of the library phage (complexity, 2.7 x 10(9)) was reacted with BacMPs. The elution of bound phages from BacMPs was performed by disrupting its membrane with phospholipase D treatment. Six candidate peptides, which were highly cationic and could bind onto the BacMP membrane, were obtained. They exhibited antimicrobial activity against Bacillus subtilis but not against Escherichia coli and Saccharomyces cerevisiae. The amino acid substitution of the selected peptide, KPQQHNRPLRHK (peptide 6-7), to enhance the hydrophobicity resulted in obvious antimicrobial activity against all test microorganisms. The present study shows for the first time that a magnetic selection of antimicrobial peptides from the phage display peptide library was successfully achieved by targeting the actual bacterial inner membrane. This BacMP-based method could be a promising approach for a high-throughput screening of antimicrobial peptides targeting a wide range of species.
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