ArticleProceedings of the National Academy of Sciences of the United States of America2008
Prediction of human miRNAs using tissue-selective motifs in 3' UTRs.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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Who cites it
8 citing papers in PubMed, 15 citations in OpenAlex.
- miRSCAPE - inferring miRNA expression from scRNA-seq data.iScience · 2022Article
- Contribution of bioinformatics prediction in microRNA-based cancer therapeutics.Advanced drug delivery reviews · 2015Review
- Fine-tuning of microRNA-mediated repression of mRNA by splicing-regulated and highly repressive microRNA recognition element.BMC genomics · 2013Article
- A Review of Computational Tools in microRNA Discovery.Frontiers in genetics · 2013Article
- Revealing the anti-tumor effect of artificial miRNA p-27-5p on human breast carcinoma cell line T-47D.International journal of molecular sciences · 2012Article
- Characterization of microRNAs from sheep (Ovis aries) using computational and experimental analyses.Molecular biology reports · 2011Article
- Evolutionary dynamics of a conserved sequence motif in the ribosomal genes of the ciliate Paramecium.BMC evolutionary biology · 2010Article
- Computational approaches for microRNA studies: a review.Mammalian genome : official journal of the International Mammalian Genome Society · 2010Review
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 3 countries.
Funding
Abstract
MicroRNAs (miRNAs) play an important role in posttranscriptional regulation of genes. We developed a method to predict human miRNAs without requiring cross-species conservation. We first identified lowly/moderately expressed tissue-selective genes using EST data and then identified overrepresented motifs of seven nucleotides in the 3' UTRs of these genes. Using these motifs as potential target sites of miRNAs, we recovered more than two-thirds of the known human miRNAs. We then used those motifs that did not match any known human miRNA seed region to infer novel miRNAs. We predicted 36 new human miRNA genes with 44 mature forms and 4 novel alternative mature forms of 2 known miRNA genes when a stringent criterion was used and many more novel miRNAs when a less stringent criterion was used. We tested the expression of 11 predicted miRNAs in three human cell lines and found 5 of them expressed in all three cell lines and 1 expressed in one cell line. We selected 2 of them, P-2 and P-27-5p, to do functional validation, using their mimics and inhibitors and using both luciferase assay and Western blotting. These experiments provided strong evidence that both P-2 and P-27-5p are novel miRNAs and that CREB3L3, which encodes cAMP-responsive element binding protein 3-like 3, is a target gene of P-2, whereas LAMB3, which encodes laminin beta3, is a target gene of P-27-5p.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.