ArticleCryobiology2009
Biophysics of zebrafish (Danio rerio) sperm.
Article in Cryobiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
12 citing papers in PubMed, 39 citations in OpenAlex.
- Interdisciplinary Tools to Safeguard and Amplify Aquatic Genetic Resource Use: A Foundation for Industrial-Scale Quality Control for Fertilization.Animals : an open access journal from MDPI · 2026Article
- Embryonic Lethality, Juvenile Growth Variation, and Adult Sterility Correlate With Phylogenetic Distance of Danionin Hybrids.Evolution & development · 2025Article
- Article
- The emerging role of open technologies for community-based improvement of cryopreservation and quality management for repository development in aquatic species.Animal reproduction science · 2022Review
- Design and Cost Analysis of a Self-contained Mobile Laboratory for Commercial-scale Aquatic Species Cryopreservation.Journal of the World Aquaculture Society · 2018Article
- A microfluidic device for motility and osmolality analysis of zebrafish sperm.Biomedical microdevices · 2018Article
- 3-D printing provides a novel approach for standardization and reproducibility of freezing devices.Cryobiology · 2017Article
- Embryogenesis and early skeletogenesis in the antarctic bullhead notothen, Notothenia coriiceps.Developmental dynamics : an official publication of the American Association of Anatomists · 2016Article
- Microfluidics and numerical simulation as methods for standardization of zebrafish sperm cell activation.Biomedical microdevices · 2015Article
- Microfluidic mixing for sperm activation and motility analysis of pearl Danio zebrafish.Theriogenology · 2012Article
- Oxidative stress in zebrafish (Danio rerio) sperm.PloS one · 2012Article
- Production of F₁ offspring with vitrified sperm from a live-bearing fish, the green swordtail Xiphophorus hellerii.Zebrafish · 2011Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 5 institutions in 1 country.
Funding
Abstract
In the past two decades, laboratories around the world have produced thousands of mutant, transgenic, and wild-type zebrafish lines for biomedical research. Although slow-freezing cryopreservation of zebrafish sperm has been available for 30 years, current protocols lack standardization and yield inconsistent post-thaw fertilization rates. Cell cryopreservation cannot be improved without basic physiological knowledge, which was lacking for zebrafish sperm. The first goal was to define basic cryobiological values for wild-type zebrafish sperm and to evaluate how modern physiological methods could aid in developing improved cryopreservation protocols. Coulter counting methods measured an osmotically inactive water fraction (Vb) of 0.37+/-0.02 (SEM), an isosmotic cell volume (V(o)) of 12.1+/-0.2 microm(3) (SEM), a water permeability (L(p)) in 10% dimethyl sulfoxide of 0.021+/-0.001(SEM)microm/min/atm, and a cryoprotectant permeability (P(s)) of 0.10+/-0.01 (SEM)x10(-3)cm/min. Fourier transform infrared spectroscopy indicated that sperm membranes frozen without cryoprotectant showed damage and lipid reorganization, while those exposed to 10% glycerol demonstrated decreased lipid phase transition temperatures, which would stabilize the cells during cooling. The second goal was to determine the practicality and viability of shipping cooled zebrafish sperm overnight through the mail. Flow cytometry demonstrated that chilled fresh sperm can be maintained at 92% viability for 24h at 0 degrees C, suggesting that it can be shipped and exchanged between laboratories. Additional methods will be necessary to analyze and improve cryopreservation techniques and post-thaw fertility of zebrafish sperm. The present study is a first step to explore such techniques.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.