ArticleMolecular reproduction and development2009
Targeted suppression of Has2 mRNA in mouse cumulus cell-oocyte complexes by adenovirus-mediated short-hairpin RNA expression.
Article in Molecular reproduction and development, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
33 citing papers in PubMed, 60 citations in OpenAlex.
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- FGF receptor 2 signaling in granulosa cells is required for normal female fertility in mice.Reproduction (Cambridge, England) · 2025Article
- Hyperandrogen-induced imbalance of FOXO4-AR regulatory loop contributes to ovulatory disorders in polycystic ovary syndrome.Molecular therapy. Nucleic acids · 2025Article
- β-hydroxybutyrate induces endoplasmic reticulum stress in bovine cumulus cells during oocyte maturationAnimal reproduction · 2025Article
- Chrysoeriol Improves the Early Development Potential of Porcine Oocytes by Maintaining Lipid Homeostasis and Improving Mitochondrial Function.Antioxidants (Basel, Switzerland) · 2024Article
- The effect of ACVR1B/TGFBR1/ACVR1C signaling inhibition on oocyte and granulosa cell development during in vitro growth culture.The Journal of reproduction and development · 2023Article
- Bovine in vitro oocyte maturation and embryo culture in liquid marbles 3D culture system.PloS one · 2023Article
- Ramelteon Reduces Oxidative Stress by Maintenance of Lipid Homeostasis in Porcine Oocytes.Antioxidants (Basel, Switzerland) · 2022Article
- Melatonin Regulates Lipid Metabolism in Porcine Cumulus-Oocyte Complexes via the Melatonin Receptor 2.Antioxidants (Basel, Switzerland) · 2022Article
- Tannin Supplementation Improves Oocyte Cytoplasmic Maturation and Subsequent Embryo Development in Pigs.Antioxidants (Basel, Switzerland) · 2021Article
- The Molecular Regulation in the Pathophysiology in Ovarian Aging.Aging and disease · 2021Review
- Up-regulated FHL2 inhibits ovulation through interacting with androgen receptor and ERK1/2 in polycystic ovary syndrome.EBioMedicine · 2020Article
- Article
- Evaluating The Effect of Melatonin on HAS2, and PGR expression, as well as Cumulus Expansion, and Fertility Potential in Mice.Cell journal · 2018Article
- Oocyte maturation, embryo development and gene expression following two different methods of bovine cumulus-oocyte complexes vitrification.Veterinary research communications · 2017Article
- Effects of exosome-like vesicles on cumulus expansion in pigs in vitro.The Journal of reproduction and development · 2017Article
- Oocyte-dependent activation of MTOR in cumulus cells controls the development and survival of cumulus-oocyte complexes.Journal of cell science · 2016Article
- Impact of Prunus Cerasus on PGR and HAS2 in Cumulus Cells and Fertility Outcome.Advanced pharmaceutical bulletin · 2016Article
- NPR2 is involved in FSH-mediated mouse oocyte meiotic resumption.Journal of ovarian research · 2016Article
- MicroRNA-378 regulates oocyte maturation via the suppression of aromatase in porcine cumulus cells.American journal of physiology. Endocrinology and metabolism · 2015Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
RNA interference (RNAi) is an effective tool for studying gene function in oocytes, but no studies have targeted somatic cells of primary cultured cumulus cell-oocyte complexes (COCs). This is probably due to difficulty in introducing RNAi-inducing molecules, such as a short-hairpin RNA (shRNA) gene, into COCs by commonly used transfection reagents. We therefore tested whether a developmental process of intact COCs could be suppressed by adenovirus-mediated shRNA expression. Has2, encoding hyaluronan synthase 2, was selected as the target transcript, because the process of cumulus expansion depends upon expression of Has2 mRNA and this process is easily evaluated in vitro. Intact COCs were infected with replication-incompetent adenoviruses containing an expression sequence of shRNA targeting either Has2 (Has2 shRNA) or a control transcript not expressed in cumulus cells, and the effects on epidermal growth factor (EGF)-stimulated cumulus expansion were determined. Has2 shRNA expression suppressed Has2 mRNA levels in COCs by more than 70%, without affecting expression levels of Ptgs2, Ptx3, Tnfaip6 mRNAs, which are also required for cumulus expansion, or other transcripts not related to expansion. Interestingly, levels of Areg and Ereg mRNAs were decreased in COCs expressing Has2 shRNA when compared with those in controls, while Btc mRNA levels remained unaffected. Furthermore, the degree of cumulus expansion by Has2 shRNA-expressing COCs was significantly less than that of controls. Thus adenovirus-mediated introduction of shRNA produces specific gene silencing and a phenotype in intact COCs, providing proof of principle that this method will be a helpful tool for understanding mechanisms of COC development.
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