ArticleCurrent biology : CB2008
An expanded biological repertoire for Ins(3,4,5,6)P4 through its modulation of ClC-3 function.
Article in Current biology : CB, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 34 citations in OpenAlex.
- Importance of Radioactive Labelling to Elucidate Inositol Polyphosphate Signalling.Topics in current chemistry (Cham) · 2017Review
- A short history of inositol lipids.Journal of lipid research · 2016Review
- Phosphoinositides: tiny lipids with giant impact on cell regulation.Physiological reviews · 2013Review
- Functional regulation of ClC-3 in the migration of vascular smooth muscle cells.Hypertension (Dallas, Tex. : 1979) · 2013Article
- Activation of PLC by an endogenous cytokine (GBP) in Drosophila S3 cells and its application as a model for studying inositol phosphate signalling through ITPK1.The Biochemical journal · 2012Article
- Toxicity mechanisms of amphotericin B and its neutralization by conjugation with arabinogalactan.Antimicrobial agents and chemotherapy · 2012Article
- Regulation of inositol 1,3,4-trisphosphate 5/6-kinase (ITPK1) by reversible lysine acetylation.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Defining signal transduction by inositol phosphates.Sub-cellular biochemistry · 2012Review
- Molecular interaction and functional regulation of ClC-3 by Ca2+/calmodulin-dependent protein kinase II (CaMKII) in human malignant glioma.The Journal of biological chemistry · 2010Article
- Regulation of immune cell development through soluble inositol-1,3,4,5-tetrakisphosphate.Nature reviews. Immunology · 2010Review
- Chloride channels: often enigmatic, rarely predictable.Annual review of physiology · 2010Review
- The Arabidopsis ATP-binding cassette protein AtMRP5/AtABCC5 is a high affinity inositol hexakisphosphate transporter involved in guard cell signaling and phytate storage.The Journal of biological chemistry · 2009Article
- The granular chloride channel ClC-3 is permissive for insulin secretion.Cell metabolism · 2009Article
- Molecular basis for the integration of inositol phosphate signaling pathways via human ITPK1.Advances in enzyme regulation · 2009Review
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6 authors at 4 institutions in 1 country.
Funding
Abstract
Ins(3,4,5,6)P(4) inhibits plasma membrane Cl(-) flux in secretory epithelia [1]. However, in most other mammalian cells, receptor-dependent elevation of Ins(3,4,5,6)P(4) levels is an "orphan" response that lacks biological significance [2]. We set out to identify Cl(-) channel(s) and/or transporter(s) that are regulated by Ins(3,4,5,6)P4 in vivo. Several candidates [3-5] were excluded through biophysical criteria, electrophysiological analysis, and confocal immunofluorescence microscopy. Then, we heterologously expressed ClC-3 in the plasma membrane of HEK293-tsA201 cells; whole-cell patch-clamp analysis showed Ins(3,4,5,6)P4 to inhibit Cl(-) conductance through ClC-3. Next, we heterologously expressed ClC-3 in the early endosomal compartment of BHK cells; by fluorescence ratio imaging of endocytosed FITC-transferrin, we recorded intra-endosomal pH, an in situ biosensor for Cl(-) flux across endosomal membranes [6]. A cell-permeant, bioactivatable Ins(3,4,5,6)P4 analog elevated endosomal pH from 6.1 to 6.6, reflecting inhibition of ClC-3. Finally, Ins(3,4,5,6)P(4) inhibited endogenous ClC-3 conductance in postsynaptic membranes of neonatal hippocampal neurones. Among other ClC-3 functions that could be regulated by Ins(3,4,5,6)P4 are tumor cell migration [7], apoptosis [8], and inflammatory responses [9]. Ins(3,4,5,6)P4 is a ubiquitous cellular signal with diverse biological actions.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.