ArticleBMC genomics2008
Enhanced cross-species utility of conserved microsatellite markers in shorebirds.
Article in BMC genomics, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
8 citing papers in PubMed, 46 citations in OpenAlex.
- Using SSR-HRM to Identify Closely Related Species in Herbal Medicine Products: A Case Study on Licorice.Frontiers in pharmacology · 2018Article
- SSR markers: a tool for species identification in Psidium (Myrtaceae).Molecular biology reports · 2015Article
- A first-generation microsatellite linkage map of the ruff.Ecology and evolution · 2013Article
- High-utility conserved avian microsatellite markers enable parentage and population studies across a wide range of species.BMC genomics · 2013Article
- Local environment but not genetic differentiation influences biparental care in ten plover populations.PloS one · 2013Article
- Triploid plover female provides support for a role of the W chromosome in avian sex determination.Biology letters · 2012Article
- Conflict between genetic and phenotypic differentiation: the evolutionary history of a 'lost and rediscovered' shorebird.PloS one · 2011Article
- Design and implementation of degenerate microsatellite primers for the mammalian clade.PloS one · 2011Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMicrosatellite markers are popular genetic markers frequently used in forensic biology. Despite their popularity, the characterisation of polymorphic microsatellite loci and development of suitable markers takes considerable effort. Newly-available genomic databases make it feasible to identify conserved genetic markers. We examined the utility and characteristics of conserved microsatellite markers in Charadriiformes (plovers, sandpipers, gulls and auks). This order harbours many species with diverse breeding systems, life histories and extraordinary migration biology whose genetics warrant investigation. However, research has been largely restrained by the limited availability of genetic markers. To examine the utility of conserved microsatellite loci as genetic markers we collated a database of Charadriiformes microsatellites, searched for homologues in the chicken genome and tested conserved markers for amplification and polymorphism in a range of charadriiform species.
resultsSixty-eight (42%) of 161 charadriiform microsatellite loci were assigned to a single location in the chicken genome based on their E-value. Fifty-five primers designed from conserved microsatellite loci with an E-value of E-10 or lower amplified across a wider range of charadriiform species than a control group of primers from ten anonymous microsatellite loci. Twenty-three of 24 examined conserved markers were polymorphic, each in on average 3 of 12 species tested.
conclusionGenomic sequence databases are useful tools to identify conserved genetic markers including those located in non-coding regions. By maximising primer sequence similarity between source species and database species, markers can be further improved and provide additional markers to study the molecular ecology of populations of non-model organisms.
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