ArticleAmerican journal of respiratory and critical care medicine2009
Endothelin-1 impairs alveolar epithelial function via endothelial ETB receptor.
Article in American journal of respiratory and critical care medicine, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 44 citations in OpenAlex.
- Macitentan: entry-into-humans study with a new endothelin receptor antagonist.European journal of clinical pharmacology · 2011Trial
- Ischemic Stroke in a Child after a Probable Scorpion Sting: Correspondence.The American journal of tropical medicine and hygiene · 2025Article
- Multicenter Validation of a Unified Evidence-Based Treatment Protocol Focusing on Clazosentan for Managing Subarachnoid Hemorrhage.Journal of clinical medicine · 2025Article
- The pathogenesis and management of heatstroke and heatstroke-induced lung injury.Burns & trauma · 2025Review
- Pediatric Ischemic Stroke Following Scorpion Sting: Correspondence.Indian journal of pediatrics · 2024Article
- Integrating Genome-Scale Metabolic Models with Patient Plasma Metabolome to Study Endothelial Metabolism In Situ.International journal of molecular sciences · 2024Article
- The Next Frontier in Lung Transplantation: Protecting the Endothelium and Repairing Organs for Transplant Utilizing MG53.Clinical and translational discovery · 2023Article
- Quercetin ameliorates acute lung injury in a rat model of hepatopulmonary syndrome.BMC complementary medicine and therapies · 2022Article
- Article
- Pharmacological Activation Of Aldehyde Dehydrogenase 2 Protects Against Heatstroke-Induced Acute Lung Injury by Modulating Oxidative Stress and Endothelial Dysfunction.Frontiers in immunology · 2021Article
- Endothelin receptor antagonist improves donor lung function in an ex vivo perfusion system.Journal of biomedical science · 2020Article
- Uncommon presentation of scorpion sting at teaching hospital.Journal of family medicine and primary care · 2020Article
- Effect of different high altitudes on vascular endothelial function in healthy people.Medicine · 2020Article
- Argon reduces the pulmonary vascular tone in rats and humans by GABA-receptor activation.Scientific reports · 2019Article
- Review
- A novel swine model of ricin-induced acute respiratory distress syndrome.Disease models & mechanisms · 2017Article
- Involvement of Cytokines in the Pathogenesis of Salt and Water Imbalance in Congestive Heart Failure.Frontiers in immunology · 2017Article
- Nur77 attenuates endothelin-1 expression via downregulation of NF-κB and p38 MAPK in A549 cells and in an ARDS rat model.American journal of physiology. Lung cellular and molecular physiology · 2016Article
- Lung Edema Clearance: Relevance to Patients with Lung Injury.Rambam Maimonides medical journal · 2015Article
- Macitentan for the treatment of pulmonary arterial hypertension.Vascular health and risk management · 2014Review
Corrections and comments
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Authors and funding
13 authors at 4 institutions in 4 countries.
Funding
Abstract
rationaleEndothelin-1 (ET-1) is increased in patients with high-altitude pulmonary edema and acute respiratory distress syndrome, and these patients have decreased alveolar fluid reabsorption (AFR).
objectivesTo determine whether ET-1 impairs AFR via activation of endothelial cells and nitric oxide (NO) generation.
methodsIsolated perfused rat lung, transgenic rats deficient in ETB receptors, coincubation of lung human microvascular endothelial cells (HMVEC-L) with rat alveolar epithelial type II cells or A549 cells, ouabain-sensitive 86Rb+ uptake. MEASUREMENTS AND MAIN
resultsThe ET-1-induced decrease in AFR was prevented by blocking the endothelin receptor ETB, but not ETA. Endothelial-epithelial cell interaction is required, as direct exposure of alveolar epithelial cells (AECs) to ET-1 did not affect Na,K-ATPase function or protein abundance at the plasma membrane, whereas coincubation of HMVEC-L and AECs with ET-1 decreased Na,K-ATPase activity and protein abundance at the plasma membrane. Exposing transgenic rats deficient in ETB receptors in the pulmonary vasculature (ET-B(-/-)) to ET-1 did not decrease AFR or Na,K-ATPase protein abundance at the plasma membrane of AECs. Exposing HMVEC-L to ET-1 led to increased NO, and the ET-1-induced down-regulation of Na,K-ATPase was prevented by the NO synthase inhibitor l-NAME, but not by a guanylate cyclase inhibitor.
conclusionsWe provide the first evidence that ET-1, via an endothelial-epithelial interaction, leads to decreased AFR by a mechanism involving activation of endothelial ETB receptors and NO generation leading to alveolar epithelial Na,K-ATPase down-regulation in a cGMP-independent manner.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.