ArticleNucleic acids research2008
The role of GTP in transient splitting of 70S ribosomes by RRF (ribosome recycling factor) and EF-G (elongation factor G).
Article in Nucleic acids research, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 24 citations in OpenAlex.
- Article
- Time resolution in cryo-EM using a novel PDMS-based microfluidic chip assembly and its application to the study of HflX-mediated ribosome recycling.bioRxiv : the preprint server for biology · 2023Article
- At the Crossroad of Nucleotide Dynamics and Protein Synthesis in Bacteria.Microbiology and molecular biology reviews : MMBR · 2023Review
- The hibernating 100S complex is a target of ribosome-recycling factor and elongation factor G inThe Journal of biological chemistry · 2020Article
- The kinetic mechanism of bacterial ribosome recycling.Nucleic acids research · 2017Article
- The conserved GTPase HflX is a ribosome splitting factor that binds to the E-site of the bacterial ribosome.Nucleic acids research · 2016Article
- New insights into the enzymatic role of EF-G in ribosome recycling.Nucleic acids research · 2015Article
- Article
- The Arabidopsis chloroplast ribosome recycling factor is essential for embryogenesis and chloroplast biogenesis.Plant molecular biology · 2010Article
- Probing translation with small-molecule inhibitors.Chemistry & biology · 2010Review
- Genome diversity of Pseudomonas aeruginosa PAO1 laboratory strains.Journal of bacteriology · 2010Article
- Distinct functions of elongation factor G in ribosome recycling and translocation.RNA (New York, N.Y.) · 2009Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
Ribosome recycling factor (RRF), elongation factor G (EF-G) and GTP split 70S ribosomes into subunits. Here, we demonstrated that the splitting was transient and the exhaustion of GTP resulted in re-association of the split subunits into 70S ribosomes unless IF3 (initiation factor 3) was present. However, the splitting was observed with sucrose density gradient centrifugation (SDGC) without IF3 if RRF, EF-G and GTP were present in the SDGC buffer. The splitting of 70S ribosomes causes the decrease of light scattering by ribosomes. Kinetic constants obtained from the light scattering studies are sufficient to account for the splitting of 70S ribosomes by RRF and EF-G/GTP during the lag phase for activation of ribosomes for the log phase. As the amount of 70S ribosomes increased, more RRF, EF-G and GTP were necessary to split 70S ribosomes. In the presence of a physiological amount of polyamines, GTP and factors, even 0.6 microM 70S ribosomes (12 times higher than the 70S ribosomes for routine assay) were split. Spermidine (2 mM) completely inhibited anti-association activity of IF3, and the RRF/EF-G/GTP-dependent splitting of 70S ribosomes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.