ArticleAnalytical chemistry2008
Thermostable llama single domain antibodies for detection of botulinum A neurotoxin complex.
Article in Analytical chemistry, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 52 citations in OpenAlex.
- Scalable, robust, high-throughput expression & purification of nanobodies enabled by 2-stage dynamic control.Metabolic engineering · 2024Article
- Nanobodies in the fight against infectious diseases: repurposing nature's tiny weapons.World journal of microbiology & biotechnology · 2024Review
- High affinity nanobodies block SARS-CoV-2 spike receptor binding domain interaction with human angiotensin converting enzyme.Scientific reports · 2020Article
- Single Domain Antibodies as New Biomarker Detectors.Diagnostics (Basel, Switzerland) · 2017Review
- Review: Microbial analysis in dielectrophoretic microfluidic systems.Analytica chimica acta · 2017Review
- Activity-based assessment of an engineered hyperthermophilic protein as a capture agent in paper-based diagnostic tests.Molecular systems design & engineering · 2016Article
- Nanomaterial-based sensors for the detection of biological threat agents.Materials today (Kidlington, England) · 2016Review
- Phage display as a promising approach for vaccine development.Journal of biomedical science · 2016Review
- Peptides in headlock--a novel high-affinity and versatile peptide-binding nanobody for proteomics and microscopy.Scientific reports · 2016Article
- Centrifugal microfluidic platform for ultrasensitive detection of botulinum toxin.Analytical chemistry · 2015Article
- Electrochemical sensing of cortisol: a recent update.Applied biochemistry and biotechnology · 2014Review
- Rugged single domain antibody detection elements for Bacillus anthracis spores and vegetative cells.PloS one · 2012Article
- Article
- Isolation of a highly thermal stable lama single domain antibody specific for Staphylococcus aureus enterotoxin B.BMC biotechnology · 2011Article
- Biotechnological applications of recombinant single-domain antibody fragments.Microbial cell factories · 2011Review
- Neutralization of Clostridium difficile toxin A with single-domain antibodies targeting the cell receptor binding domain.The Journal of biological chemistry · 2011Article
- Llama-derived single-domain antibodies for the detection of botulinum A neurotoxin.Analytical and bioanalytical chemistry · 2010Article
- Llama single domain antibodies specific for the 7 botulinum neurotoxin serotypes as heptaplex immunoreagents.PloS one · 2010Article
- Single domain antibodies: promising experimental and therapeutic tools in infection and immunity.Medical microbiology and immunology · 2009Review
- Ricin detection using phage displayed single domain antibodies.Sensors (Basel, Switzerland) · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
Immunoglobulins from animals of the Camelidae family boast unique forms that do not incorporate light chains. Antigen binding in these unconventional heavy-chain homodimers is mediated through a single variable domain. When expressed recombinantly these variable domains are termed single domain antibodies (sdAb) and are among the smallest naturally IgG-derived antigen binding units. SdAb possess good solubility, thermostability, and can refold after heat and chemical denaturation making them promising alternative recognition elements. We have constructed a library of phage-displayed sdAb from a llama immunized with a cocktail of botulinum neurotoxin (BoNT) complex toxoids and panned the library for binders for BoNT A complex toxoid. Six unique binders were isolated and found to specifically bind BoNT A complex in toxoid and untoxoided forms and when used in optimal combinations in buffer and milk could detect 100 pg/mL untoxoided complex. All sdAb retained their ability to specifically bind target after heating to 85 degrees C for 1 h, in contrast to conventional polyclonal sera. All of the sdAb were highly specific for subtype A1 rather than A2 and demonstrated binding to the 33 kDa hemagglutinin, potentially to a somewhat overlapping linear epitope. The unique properties of these sdAb may provide advantages for many diagnostic applications where long-term storage and in-line monitoring require very rugged yet highly specific recognition elements.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.