ArticleMolecular biology of the cell2009
A lipid-signaled myosin phosphatase surge disperses cortical contractile force early in cell spreading.
Article in Molecular biology of the cell, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 36 citations in OpenAlex.
- PILS-Nir1 is a sensitive phosphatidic acid biosensor that reveals mechanisms of lipid production.The Journal of cell biology · 2025Article
- PILS-Nir1 is a novel phosphatidic acid biosensor that reveals mechanisms of lipid production.bioRxiv : the preprint server for biology · 2025Article
- Phosphatidic acid signaling in modulating plant reproduction and architecture.Plant communications · 2025Review
- Phosphatidic acid signaling and function in nuclei.Progress in lipid research · 2024Review
- Optogenetic relaxation of actomyosin contractility uncovers mechanistic roles of cortical tension during cytokinesis.Nature communications · 2021Article
- Phosphatidic acid increases Notch signalling by affecting Sanpodo trafficking during Drosophila sensory organ development.Scientific reports · 2020Article
- Measuring Phospholipase D Enzymatic Activity Through Biochemical and Imaging Methods.Methods in enzymology · 2017Article
- Phospholipase D1-regulated autophagy supplies free fatty acids to counter nutrient stress in cancer cells.Cell death & disease · 2016Article
- Membrane Tension Acts Through PLD2 and mTORC2 to Limit Actin Network Assembly During Neutrophil Migration.PLoS biology · 2016Article
- Role of mitochondrial lipids in guiding fission and fusion.Journal of molecular medicine (Berlin, Germany) · 2015Review
- Visualization of phosphatidic acid fluctuations in the plasma membrane of living cells.PloS one · 2014Article
- Temporal production of the signaling lipid phosphatidic acid by phospholipase D2 determines the output of extracellular signal-regulated kinase signaling in cancer cells.Molecular and cellular biology · 2014Article
- EDI3 links choline metabolism to integrin expression, cell adhesion and spreading.Cell adhesion & migration · 2014Article
- Diacylglycerol kinases terminate diacylglycerol signaling during the respiratory burst leading to heterogeneous phagosomal NADPH oxidase activation.The Journal of biological chemistry · 2013Article
- Phosphatidic acid is required for the constitutive ruffling and macropinocytosis of phagocytes.Molecular biology of the cell · 2013Article
- Article
- Evidence for two CRIB domains in phospholipase D2 (PLD2) that the enzyme uses to specifically bind to the small GTPase Rac2.The Journal of biological chemistry · 2011Article
- Heterogeneity of phosphatidic acid levels and distribution at the plasma membrane in living cells as visualized by a Föster resonance energy transfer (FRET) biosensor.The Journal of biological chemistry · 2010Article
- Protein kinase Cdelta-mediated phosphorylation of phospholipase D controls integrin-mediated cell spreading.Molecular and cellular biology · 2010Article
- Basis for the isoform-specific interaction of myosin phosphatase subunits protein phosphatase 1c beta and myosin phosphatase targeting subunit 1.The Journal of biological chemistry · 2010Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
When cells cease migrating through the vasculature, adhere to extracellular matrix, and begin to spread, they exhibit rapid changes in contraction and relaxation at peripheral regions newly contacting the underlying substrata. We describe here a requirement in this process for myosin II disassembly at the cell cortex via the action of myosin phosphatase (MP), which in turn is regulated by a plasma membrane signaling lipid. Cells in suspension exhibit high levels of activity of the signaling enzyme phospholipase D2 (PLD2), elevating production of the lipid second messenger phosphatidic acid (PA) at the plasma membrane, which in turn recruits MP and stores it there in a presumed inactive state. On cell attachment, down-regulation of PLD2 activity decreases PA production, leading to MP release, myosin dephosphorylation, and actomyosin disassembly. This novel model for recruitment and restraint of MP provides a means to effect a rapid cytoskeletal reorganization at the cell cortex upon demand.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.