ArticleClinical and vaccine immunology : CVI2008
Real-time in vivo green fluorescent protein imaging of a murine leishmaniasis model as a new tool for Leishmania vaccine and drug discovery.
Article in Clinical and vaccine immunology : CVI, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
24 citing papers in PubMed, 46 citations in OpenAlex.
- Article
- Reporter gene systems: A powerful tool forCurrent research in microbial sciences · 2022Article
- Exogenous Signaling Molecules Released from Aptamer-Functionalized Hydrogels Promote the Survival of Mesenchymal Stem Cell Spheroids.ACS applied materials & interfaces · 2020Article
- Visualization of Leishmania tropica Infection in BALB/c Mice by Bioluminescence ImagingIranian biomedical journal · 2020Article
- Route map for the discovery and pre-clinical development of new drugs and treatments for cutaneous leishmaniasis.International journal for parasitology. Drugs and drug resistance · 2019Review
- Generation and Characterization of a Dual-Reporter TransgenicFrontiers in cellular and infection microbiology · 2019Article
- Use of Optical Imaging Technology in the Validation of a New, Rapid, Cost-Effective Drug Screen as Part of a TieredAntimicrobial agents and chemotherapy · 2017Article
- Infrared fluorescent imaging as a potent tool for in vitro, ex vivo and in vivo models of visceral leishmaniasis.PLoS neglected tropical diseases · 2015Article
- A Review: The Current In Vivo Models for the Discovery and Utility of New Anti-leishmanial Drugs Targeting Cutaneous Leishmaniasis.PLoS neglected tropical diseases · 2015Review
- Expression of fluorescent proteins in bifidobacteria for analysis of host-microbe interactions.Applied and environmental microbiology · 2014Article
- In vitro screening test using Leishmania promastigotes stably expressing mCherry protein.Antimicrobial agents and chemotherapy · 2014Article
- An alternative in vitro drug screening test using Leishmania amazonensis transfected with red fluorescent protein.Diagnostic microbiology and infectious disease · 2013Article
- Stage-regulated GFP Expression in Trypanosoma cruzi: applications from host-parasite interactions to drug screening.PloS one · 2013Article
- Bacterial vectors for imaging and cancer gene therapy: a review.Cancer gene therapy · 2012Review
- Appraisal of a Leishmania major strain stably expressing mCherry fluorescent protein for both in vitro and in vivo studies of potential drugs and vaccine against cutaneous leishmaniasis.PLoS neglected tropical diseases · 2012Article
- Use of in vivo and in vitro systems to select Leishmania amazonensis expressing green fluorescent protein.The Korean journal of parasitology · 2011Article
- Luciferase-expressing Leishmania infantum allows the monitoring of amastigote population size, in vivo, ex vivo and in vitro.PLoS neglected tropical diseases · 2011Article
- C-terminal domain deletion enhances the protective activity of cpa/cpb loaded solid lipid nanoparticles against Leishmania major in BALB/c mice.PLoS neglected tropical diseases · 2011Article
- Noninvasive biophotonic imaging for studies of infectious disease.FEMS microbiology reviews · 2011Review
- Visceral leishmaniasis: experimental models for drug discovery.The Indian journal of medical research · 2011Review
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 1 country.
Funding
Abstract
Leishmania species are obligate intracellular protozoan parasites that cause a broad spectrum of clinical diseases in mammalian hosts. The most frequently used approach to quantify parasites in murine model systems is based on thickness measurements of the footpad or ear after experimental infection. To overcome the limitations of this method, we used a Leishmania mutant episomally transfected with enhanced green fluorescent protein, enabling in vivo real-time whole-body fluorescence imaging, to follow the progression of Leishmania infection in parasitized tissues. Fluorescence correlated with the number of Leishmania parasites in the tissue and demonstrated the real-time efficacy of a therapeutic vaccine. This approach provides several substantial advantages over currently available animal model systems for the in vivo study of immunopathogenesis, prevention, and therapy of leishmaniasis. These include improvements in sensitivity and the ability to acquire real-time data on progression and spread of the infection.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.