ArticleClinical and vaccine immunology : CVI2008
Evaluation of antigen-specific T-cell responses with a miniaturized and automated method.
Article in Clinical and vaccine immunology : CVI, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 17 citations in OpenAlex.
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- Article
- The TB-specific CD4(+) T cell immune repertoire in both cynomolgus and rhesus macaques largely overlap with humans.Tuberculosis (Edinburgh, Scotland) · 2015Article
- Miniaturized and high-throughput assays for analysis of T-cell immunity specific for opportunistic pathogens and HIV.Clinical and vaccine immunology : CVI · 2014Article
- High throughput T epitope mapping and vaccine development.Journal of biomedicine & biotechnology · 2010Review
- Antigen-specific B cell detection reagents: use and quality control.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2008Review
- The curation guidelines of the immune epitope database and analysis resource.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2008Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The evaluation of antigen-specific T-cell responses is helpful for both research and clinical settings. Several techniques can enumerate antigen-responsive T cells or measure their products, but they require remarkable amounts of peripheral blood mononuclear cells (PBMCs). Since screening numerous antigens or testing samples from pediatric or lymphopenic patients is hampered in clinical practice, we refined a miniaturized, high-throughput assay for T-cell immunity. Antigens and cells in 10-microl volumes were dispensed into 1,536-well culture plates precoated with anti-gamma interferon (anti-IFN-gamma) antibodies. After being cultured, the wells were developed by enzyme-linked immunosorbent assay for bound cytokine. Miniaturization and automation allowed quantitation of antigen-specific responses on 10(4) PBMCs. This method was applied for epitope mapping of mycobacterial antigens and was used in the clinic to evaluate T-cell immunity to relevant opportunistic pathogens by using small blood samples. A comparison with conventional methods showed similar sensitivity. Therefore, current flow cytometric methods that provide information on frequency and phenotype of specific T cells can be complemented by this assay that provides extensive information on cytokine concentrations and profiles and requires 20- to 50-fold fewer PBMCs than other analytical methods.
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Registered trials
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