ArticleRNA (New York, N.Y.)2008
Stabilization of RNA during laser capture microdissection by performing experiments under argon atmosphere or using ethanol as a solvent in staining solutions.
Article in RNA (New York, N.Y.), 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers, 1 of them a synthesis that pooled it.
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Who cites it
43 citing papers in PubMed, 1 synthesis or guideline pooled it, 82 citations in OpenAlex.
- Molecular analysis of H&E- and Papanicolau-stained samples-systematic review.Histochemistry and cell biology · 2020Pooled it
- A high-quality RNA-yielding protocol for laser capture microdissection of transplanted stem cell-derived Islets of Langerhans.Acta diabetologica · 2026Article
- Immuno-laser capture microdissection of perfusion-fixed mouse brain tissue coupled to RNA-seq.Journal of neuroscience methods · 2025Article
- Intestinal Microbiota and Fecal Transplantation in Patients with Inflammatory Bowel Disease andJournal of clinical medicine · 2025Review
- Optimized protocol to preserve RNA integrity for laser capture microdissection of bovine mammary epithelial cells.JDS communications · 2025Article
- DAMPs Drive Fibroinflammatory Changes in the Glaucomatous ONH.Investigative ophthalmology & visual science · 2024Article
- Scoping Review: Methods and Applications of Spatial Transcriptomics in Tumor Research.Cancers · 2024Article
- Hybridization Chain Reaction for mRNA Localization in Single Cells from Mouse and Human Cryosections.Current protocols · 2022Article
- Single-cell sequencing of human midbrain reveals glial activation and a Parkinson-specific neuronal state.Brain : a journal of neurology · 2022Article
- Human induced pluripotent stem cells integrate, create synapses and extend long axons after spinal cord injury.Journal of cellular and molecular medicine · 2022Article
- Somatic regulation of female germ cell regeneration and development in planarians.Cell reports · 2022Article
- Early life exposure to nicotine modifies lung gene response after elastase-induced emphysema.Respiratory research · 2022Article
- Therapeutic downregulation ofeLife · 2022Article
- Robust Acquisition of Spatial Transcriptional Programs in Tissues With Immunofluorescence-Guided Laser Capture Microdissection.Frontiers in cell and developmental biology · 2022Article
- Infection cushions of Fusarium graminearum are fungal arsenals for wheat infection.Molecular plant pathology · 2020Article
- Laser Capture Microdissection of Glioma Subregions for Spatial and Molecular Characterization of Intratumoral Heterogeneity, Oncostreams, and Invasion.Journal of visualized experiments : JoVE · 2020Article
- An Optimised Protocol Harnessing Laser Capture Microdissection for Transcriptomic Analysis on Matched Primary and Metastatic Colorectal Tumours.Scientific reports · 2020Article
- Informing disease modelling with brain-relevant functional genomic annotations.Brain : a journal of neurology · 2019Review
- Validation of Laser Capture Microdissection Protocol in Endometriosis Studies.Medicina (Kaunas, Lithuania) · 2019Article
- Ischemic axonal injury up-regulates MARK4 in cortical neurons and primes tau phosphorylation and aggregation.Acta neuropathologica communications · 2019Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The combination of laser capture microdissection (LCM) and gene expression experiments allows cell specific expression profiling, which is decisive in cellular transcriptomic exploration. LCM makes possible the isolation of unique cells or group of cells, but maintaining RNA quality during this process is challenging. Several protocols are available for section preparation, but none of those guarantees the integrity of the RNA during microdissection, and operators are recommended to perform LCM during a limited time. We hypothesized that the cause of RNA degradation during the microdissection time is the presence of water rendering endogenous RNase activity possible. We thus developed two methods that stabilize RNA during microdissection time for up to 90 min. The first one consists of performing LCM under an argon atmosphere, thus preventing tissue rehydration; it is compliant with all existing microdissection protocols. The second one is a new fixation and staining method using ethanol as solvent in all preparatory steps to LCM that enhances fixation and dehydration of samples. We assessed several stains in regard of their effect on tissue morphology and RNA integrity and adjusted an ethanolic staining solution of cresyl violet and eosin Y.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.