ArticlePloS one2008
Diaphanous-related formin 2 and profilin I are required for gastrulation cell movements.
Article in PloS one, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 47 citations in OpenAlex.
- Identification of a Novel Likely Pathogenic Variant of DIAPH3 Associated With New Phenotype of Sensorineural Hearing Loss.Molecular genetics & genomic medicine · 2026Article
- Profilin and Non-Canonical Wnt Signaling: Coordinating Cytoskeletal Dynamics from Development to Disease.Journal of developmental biology · 2025Review
- Reversion induced LIM domain protein (RIL) is a Daam1-interacting protein and regulator of the actin cytoskeleton during non-canonical Wnt signaling.Developmental biology · 2024Article
- Review
- Tools of the trade: studying actin in zebrafish.Histochemistry and cell biology · 2020Review
- Disruption of profilin1 function suppresses developmental and pathological retinal neovascularization.The Journal of biological chemistry · 2020Article
- Wnt5b integrates Fak1a to mediate gastrulation cell movements via Rac1 and Cdc42.Open biology · 2020Article
- Cellular and molecular mechanisms of convergence and extension in zebrafish.Current topics in developmental biology · 2020Review
- Profilin choreographs actin and microtubules in cells and cancer.International review of cell and molecular biology · 2020Article
- Article
- Models of convergent extension during morphogenesis.Wiley interdisciplinary reviews. Developmental biology · 2018Review
- Cell adhesion molecule control of planar spindle orientation.Cellular and molecular life sciences : CMLS · 2016Review
- Human Kidney Disease-causing INF2 Mutations Perturb Rho/Dia Signaling in the Glomerulus.EBioMedicine · 2014Article
- The actin regulators Enabled and Diaphanous direct distinct protrusive behaviors in different tissues during Drosophila development.Molecular biology of the cell · 2014Article
- Formins as effector proteins of Rho GTPases.Small GTPases · 2014Review
- Formin' cellular structures: Physiological roles of Diaphanous (Dia) in actin dynamics.Communicative & integrative biology · 2013Review
- Protein phosphatase 1 β paralogs encode the zebrafish myosin phosphatase catalytic subunit.PloS one · 2013Article
- Formins at a glance.Journal of cell science · 2013Review
- Anthrax toxin receptor 2a controls mitotic spindle positioning.Nature cell biology · 2013Article
- Transcriptional regulation of Profilin during wound closure in Drosophila larvae.Journal of cell science · 2012Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Intensive cellular movements occur during gastrulation. These cellular movements rely heavily on dynamic actin assembly. Rho with its associated proteins, including the Rho-activated formin, Diaphanous, are key regulators of actin assembly in cellular protrusion and migration. However, the function of Diaphanous in gastrulation cell movements remains unclear. To study the role of Diaphanous in gastrulation, we isolated a partial zebrafish diaphanous-related formin 2 (zdia2) clone with its N-terminal regulatory domains. The GTPase binding domain of zDia2 is highly conserved compared to its mammalian homologues. Using a yeast two-hybrid assay, we showed that zDia2 interacts with constitutively-active RhoA and Cdc42. The zdia2 mRNAs were ubiquitously expressed during early embryonic development in zebrafish as determined by RT-PCR and whole-mount in situ hybridization analyses. Knockdown of zdia2 by antisense morpholino oligonucleotides (MOs) blocked epiboly formation and convergent extension in a dose-dependent manner, whereas ectopic expression of a human mdia gene partially rescued these defects. Time-lapse recording further showed that bleb-like cellular processes of blastoderm marginal deep marginal cells and pseudopod-/filopod-like processes of prechordal plate cells and lateral cells were abolished in the zdia2 morphants. Furthermore, zDia2 acts cell-autonomously since transplanted zdia2-knockdown cells exhibited low protrusive activity with aberrant migration in wild type host embryos. Lastly, co-injection of antisense MOs of zdia2 and zebrafish profilin I (zpfn 1), but not zebrafish profilin II, resulted in a synergistic inhibition of gastrulation cell movements. These results suggest that zDia2 in conjunction with zPfn 1 are required for gastrulation cell movements in zebrafish.
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