ArticleNucleic acids research2008
NF-Y substitutes H2A-H2B on active cell-cycle promoters: recruitment of CoREST-KDM1 and fine-tuning of H3 methylations.
Article in Nucleic acids research, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It has been retracted, and should not be counted. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 34 citations in OpenAlex.
- Epigenetics and cell cycle regulation in cystogenesis.Cellular signalling · 2020Review
- Silencing of LSD1 gene modulates histone methylation and acetylation and induces the apoptosis of JeKo-1 and MOLT-4 cells.International journal of molecular medicine · 2017Article
- Prognostic role of LSD1 in various cancers: evidence from a meta-analysis.OncoTargets and therapy · 2015Article
- Uncovering enhancer functions using the α-globin locus.PLoS genetics · 2014Review
- Effects of the monoamine oxidase inhibitors pargyline and tranylcypromine on cellular proliferation in human prostate cancer cells.Oncology reports · 2013Article
- LSD1-mediated epigenetic modification contributes to proliferation and metastasis of colon cancer.British journal of cancer · 2013Article
- Immune mediators regulate CFTR expression through a bifunctional airway-selective enhancer.Molecular and cellular biology · 2013Article
- Positive expression of LSD1 and negative expression of E-cadherin correlate with metastasis and poor prognosis of colon cancer.Digestive diseases and sciences · 2013Article
- FAD-dependent lysine-specific demethylase-1 regulates cellular energy expenditure.Nature communications · 2012Article
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- Identification and characterization of NF-Y transcription factor families in the monocot model plant Brachypodium distachyon.PloS one · 2011Article
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- Genome-wide analysis of regions similar to promoters of histone genes.BMC systems biology · 2010Article
- Alternative splicing of the histone demethylase LSD1/KDM1 contributes to the modulation of neurite morphogenesis in the mammalian nervous system.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2010Article
- Small molecule regulators of Rb-E2F pathway as modulators of transcription.Biochimica et biophysica actaReview
Corrections and comments
- Retraction · 2013-08-01Error in Image · Error in Methods ·
- Retracted
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The CCAAT box is a frequent promoter element, as illustrated by bioinformatic analysis, and it is bound by NF-Y, a trimer with H2A-H2B-like subunits. We developed a MNase I-based ChIP protocol on homogeneous cell populations to study cell-cycle promoters at the single nucleosome level. We analyzed histone methylations and the association of enzymatic activities. Two novel results emerged: (i) H3-H4 are present on core promoters under active conditions, with the expected cohort of 'positive' modifications; H2A-H2B are removed and substituted by NF-Y. Through the use of a dominant negative mutant we show that NF-Y is important for H3K36me3 deposition and for elongation, not recruitment of Pol II; (ii) H3K4 methylations are highly dynamic and H3K4me1 is a crucial positive mark. Functional siRNA inactivation and treatment with Tranylcypromine determined that KDM1 (LSD1) plays a positive role in transcription, specifically of G2/M genes. It requires CoREST, which is recruited on active promoters through direct interactions with NF-Y. These data are the first in vivo indication of a crucial interplay between core histones and 'deviant' histone-fold such as NF-Y, leading to fine-tuning of histone methylations.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.