ArticleStructure (London, England : 1993)2008
Recognition of the activated states of Galpha13 by the rgRGS domain of PDZRhoGEF.
Article in Structure (London, England : 1993), 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 49 citations in OpenAlex.
- Deciphering a mechanistic basis for the pathological effect of the GNAO1 E246K variant in neurodevelopmental disorder.BBA advances · 2026Article
- Stabilization of interdomain closure by a G protein inhibitor.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Interaction kinetics between p115-RhoGEF and GαCommunications biology · 2022Article
- Article
- A network of GαScience signaling · 2022Article
- Revealing the Activity of Trimeric G-proteins in Live Cells with a Versatile Biosensor Design.Cell · 2020Article
- Probing the mutational landscape of regulators of G protein signaling proteins in cancer.Science signaling · 2020Article
- Direct regulation of p190RhoGEF by activated Rho and Rac GTPases.Journal of structural biology · 2018Article
- Tyrosine phosphorylation switching of a G protein.The Journal of biological chemistry · 2018Article
- Mechanism of the intrinsic arginine finger in heterotrimeric G proteins.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- A Gα12-specific Binding Domain in AKAP-Lbc and p114RhoGEF.Journal of molecular signaling · 2016Article
- Invited review: Activation of G proteins by GTP and the mechanism of Gα-catalyzed GTP hydrolysis.Biopolymers · 2016Review
- Probing Gαi1 protein activation at single-amino acid resolution.Nature structural & molecular biology · 2015Article
- Gastrin-stimulated Gα13 Activation of Rgnef Protein (ArhGEF28) in DLD-1 Colon Carcinoma Cells.The Journal of biological chemistry · 2015Article
- Gα13/PDZ-RhoGEF/RhoA signaling is essential for gastrin-releasing peptide receptor-mediated colon cancer cell migration.Molecular pharmacology · 2014Article
- Regulated localization is sufficient for hormonal control of regulator of G protein signaling homology Rho guanine nucleotide exchange factors (RH-RhoGEFs).The Journal of biological chemistry · 2014Article
- Phosphorylation-mediated regulation of GEFs for RhoA.Cell adhesion & migration · 2014Review
- Activated RhoA is a positive feedback regulator of the Lbc family of Rho guanine nucleotide exchange factor proteins.The Journal of biological chemistry · 2013Article
- Determinants at the N- and C-termini of Gα12 required for activation of Rho-mediated signaling.Journal of molecular signaling · 2013Article
- Structural and functional analysis of the regulator of G protein signaling 2-gαq complex.Structure (London, England : 1993) · 2013Article
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5 authors at 2 institutions in 1 country.
Funding
Abstract
G12 class heterotrimeric G proteins stimulate RhoA activation by RGS-RhoGEFs. However, p115RhoGEF is a GTPase Activating Protein (GAP) toward Galpha13, whereas PDZRhoGEF is not. We have characterized the interaction between the PDZRhoGEF rgRGS domain (PRG-rgRGS) and the alpha subunit of G13 and have determined crystal structures of their complexes in both the inactive state bound to GDP and the active states bound to GDP*AlF (transition state) and GTPgammaS (Michaelis complex). PRG-rgRGS interacts extensively with the helical domain and the effector-binding sites on Galpha13 through contacts that are largely conserved in all three nucleotide-bound states, although PRG-rgRGS has highest affinity to the Michaelis complex. An acidic motif in the N terminus of PRG-rgRGS occupies the GAP binding site of Galpha13 and is flexible in the GDP*AlF complex but well ordered in the GTPgammaS complex. Replacement of key residues in this motif with their counterparts in p115RhoGEF confers GAP activity.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.